Erythrocyte Methaemoglobin Concentration and Methaemoglobin Reductase Activity in the Presence of Nevirapine and Efavirenzin Wistar Rats
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2015, Vol 3, Issue 2
Abstract
The effects of nevirapine and efavirenz on methaemoglobin concentration and methaemoglobin reductase activity on wistar rats were investigated. Ninety rats with average body weight 100 grams were allowed to acclimatize for two weeks and were distributed into two groups. Drugs at dosage 0.2, 0.4, 0.6 and 0.8mg/l00g body weightof nevirapine; and 0.6, 0.8, 1.0 and 1.2mg/l00g body weightof efavirenz were administered orally by intubation. Blood samples were collected from the experimental animals by cardiac puncture. Spectrophometric methods were used to ascertain the methhaemoglobin concentrations and the activity of NADH methaemoglobin reductase (NADH MR). The results revealed that both drugs increased methaemoglobin concentrations. The increase from 1.50±0.00 to 2.22±0.04 on the 21st day in the presence of nevirapine was significant (p<0.05), while efavirenz cause a non-significant increase throughout the experiment. Methaemoglobin reductase activity decreased significantly (p<0.05) from 5.46±0.80 to 4.74±1.09, 5.51±1.03 to 4.74±0.15 and 5.48±0.11 to 4.73±1.03 in the 7th , 14th and 21st days respectively week respectively in the presence of nevirapine, while the decrease due to efavirenz was not significant (p<0.05). Thus, to an extent both drugs adversely affected erythrocytes’s ability to maintain haemoglobin in its oxygen carrying state. Keywords: Antiretroviral drugs Nevirapine, efavirenz, methaemoglobin concentration, methaemoglobin reductase, Erythrocyte
Authors and Affiliations
Uwakwe, . A. A, Wodu, E. , Monanu . , M. O.
Extended Spectrum B-Lactamases among Clinical Isolates of Enterobacteriaceae Ssp.: Prevalence and Susceptibility Pattern at A Tertiary Care Hospital
The purpose of this study was to know prevalence and susceptibility pattern of extended spectrum β-lactamases(ESBLs) at our tertiary care centre. A total of 660 non-repetitive clinical isolates of Enterobacteriaceae obt...
The Association between Oral Health Habits and Quality of Life for Residents of a Psychiatric Nursing Home in Southern Taiwan
Abstract: The issue of daily care for patients with chronic mental disorders has recently been given more consideration. Many studies have revealed a strong association between oral diseases and chronic diseases. There i...
Seroprevalence of C - Reactive Protein in Rheumatoid Arthritis Cases: A Retrospective Study in a Tertiary Care Hospital in South India
Abstract: Rheumatoid arthritis (RA) is a chronic, systemic, autoimmune inflammatory disease that affects mainly the small joints of the hands and feet. It is one of the most common inflammatory joint diseases that compro...
Diagnosis of Osteopenia and Osteoporosis by Dual Energy X-Ray Absorptiometry
Abstract:This was analytic descriptive study. The aim of this study was to assess the role of the Dual Energy X-ray Absorptiometry scan (DEXA) for lumbar spine and hip joint in diagnosis of osteopenia and osteoprosis. Th...
Superior Mesenteric Artery Responses for Variant Meals by Color Doppler
Duplex ultrasound (DU) is ultrasound techniques that provide a simple, portable, reproducible, and non-invasive assessment of blood flow. Doppler sonography is also the most affordable method to measure noninvasively the...