Establishing reference values and evaluation of an in-house ferric reducing antioxidant power (FRAP) colorimetric assay in microplates
Journal Title: The European Research Journal - Year 2016, Vol 2, Issue 2
Abstract
Objective. The total antioxidant capacity (TAC) of a sample can be measured with a ferric reducing antioxidant power (FRAP) assay. There are commercially available kits for FRAP assays, however they are more expensive than in-house kits. We aimed to evaluate a FRAP direct measurement method under our laboratory conditions using a microplate reader and establish reference values to use in future research projects. Methods. An in-house microplate adaptation of the FRAP method was evaluated. Reference values of FRAP were established for one hundred and twenty subjects aged between 25-55 years. FRAP levels were estimated in 30 serum samples with high glucose concentration, 44 hyperbiluribinemic neonatals and 16 patients receiving renal replacement therapy (RRT). Results. The mean FRAP level was 890±235 µmol/L. The median TAC level was 904 µmol/L. This method was found to be linear up to at least 2000 µmol/L. The intra- and inter-assay coefficients of variation were 2.7-6.7% and 5.3-10.1%, respectively. The mean FRAP level was lower than normal in diabetes and RRT patients and higher in hyperbiluribinemic neonatals (687±209 µmol/L, 609±250 µmol/L and 945±187 µmol/L, respectively). Conclusions. Our reference values give comparable results with the literature. This method is simple, reliable, and inexpensive. It could be used for studies of oxidative stress-related diseases.
Authors and Affiliations
Yasemin Ustundag, Kagan Huysal, Serdar Kahvecioglu, Hakan Demirci, Senol Yavuz, Murat Sambel, Dursun Unal
Keywords
Related Articles
- EP ID EP183109
- DOI 10.18621/eurj.2016.5000190265
- Views 221
- Downloads 0
Discrete subaortic stenosis in an adult patient
Discrete subaortic stenosis is an unusual cause of the left ventricular outflow tract obstruction in the adults and characterized by a discrete subaortic membrane. A 52-year-old female patient presented with chief compla...
Spontaneous uvula hematoma: an unusual case
Hematomas in upper aerodigestive tract are not rare especially in patients with cardiologic conditions.Without comorbidities, it can also be seen after trauma cases. Sponatenous hematoma is a rare clinical condition that...
The relationship between atrial natriuretic peptide and microvascular complications of diabetes
Objective: In this study, we aimed to determine the relationship between the atrial natriuretic peptide and microvascular complications of diabetes. Methods: Sixty patients with type 2 diabetes mellitus were enrolled in...
A pregnant woman with primary hyperparathyroidism: a case report
Primary hyperparathyroidism is diagnosed with an increase of calcium levels and a decrease in phosphorus levels. Primary hyperparathyroidism is a rare condition in pregnancy. Calcium levels tend to be low in pregnant wom...
Evaluation of dry eye-associated symptoms and signs after microincision cataract surgery
Objectives. To evaluate the effects of microincision cataract surgery on dry eye-associated symptoms and signs. Methods. This prospective study included 40 eyes of 32 patients. Microincision cataract surgery was performe...