Establishment and application of a retinal ganglion cell-specific reporter stem cell line
Journal Title: Eye Science (Yanke Xuebao) - Year 2022, Vol 37, Issue 4
Abstract
[Objective:] This study was designed to establish a mouse embryonic stem cell line that can drive GFP expression specifically in retinal ganglion cells (RGCs). [Methods:] In this study, we established a Brn3b-GFP knock-in embryonic stem cell line (Brn3b-GFP ESC) by homologous recombination. By 3D culture, we induced these cells into retinal organoids to investigate the cell-specificity of GFP expression. GFP-positive RGCs were then enriched by flow cytometry and transplanted by intravitreal injection into the eyes of healthy mice and NMDA injury model mice to explore the feasibility of a potential clinical application. [Results:] GFP was specifically expressed in RGCs following induction of Brn3b-GFP ESCs into 3D retinal organoids. Two weeks after these GFP-positive RGCs were transplanted into the control and injured mice, GFP-positive cells were observed in all transplanted retinas, and donor RGCs were seen to integrate into the RGC layer of the host retina. [Conclusion:] This study has established a retinal ganglion cell-specific reporter stem cell line Brn3b-GFP ESC. The GFP-positive RGCs obtained by inducing the cell line into retinal organoids can be integrated into the host retina after transplantation. The establishment of such a cell line will provide an important research tool for glaucoma and related diseases.
Authors and Affiliations
Rong ZHANG, Fa YUAN, Mengqing XIANG
Keywords
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- EP ID EP763649
- DOI 10.3978/j.issn.1000-4432.2022.02.04
- Views 17
- Downloads 0
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