Establishment of an animal model of no-reflow ischemic stroke and the multidimensional evaluation system
Journal Title: Chinese Journal of Nervous and Mental Diseases - Year 2024, Vol 50, Issue 1
Abstract
[Objective] The purpose of this study was to explore a suitable method to model no-reflow phenomenon following ischemic stroke and to evaluate perfusion decrease from multiple perspectives. [Methods] Laser scatter contrast imaging and two-photon live imaging were used to compare transient middle cerebral artery occlusion in C57BL/6 and BALB/c mice and perfusion alterations in BALB/c mice with 1 or 1.5 h of ischemia. Several imaging techniques including laser scatter contrast imaging, low and higher magnification images of perfused brain slices and two-photon microscopy to monitor erythrocyte flow rate and flux were used to assess in vivo dynamics as well as whole brain sections and microvasculature for decreased cerebral perfusion after transient middle cerebral artery occlusion. Infarct size and behavioral deficits were assessed with microtubule-associated protein 2 staining and behavioral scoring. [Results] In C57BL/6 mice, most capillaries in the middle cerebral artery region remained flowing during ischemia, whereas most capillaries were blocked in BALB/c mice. In addition, cortical perfusion at 24 h of recanalization was significantly reduced to 76.1% of baseline following 1.5 h of ischemia in BALB/c mice (P=0.046 compared with the sham group), whereas for it was reduced to 79.9% following 1h of ischemia which was not significantly different from the sham group (P=0.299). Transient middle cerebral artery occlusion in BALB/c mice for 1.5 h resulted in a reduction in whole-brain perfusion to 75.1% (P<0.001 compared with the sham group), and erythrocyte flow rate assessed by two-photon live-imaging of erythrocyte flow on the cortical surface of the middle cerebral artery basin was reduced to 50.3% of baseline levels at 24 h of recanalization (P=0.010 compared with the sham group ), and erythrocyte flux decreased to 38.9% of baseline levels (P=0.010 compared with the sham group); high-magnification imaging of sections assessed an approximately 76% reduction in the length of capillaries with perfusion (P=0.0001 compared with the sham group), and a reduction in the fraction of the total volume occupied by perfused capillaries by an approximately 76% reduction (P<0.001 compared with the sham-operated group). Microtubule-associated protein 2 staining suggested that transient middle cerebral artery occlusion for 1.5 h in BALB/c mice resulted in infarcts that accounted for approximately 36% of the total cerebral area and behavioral scores elevated to 9, suggesting behavioral deficits. [Conclusion] Transient ischemia in BALB/c mice for 1.5 h resulted in a significant decrease in cerebral perfusion as well as capillary no-reflow and thus can model the no-reflow phenomenon following ischemic stroke. The combination of laser scatter contrast imaging, low magnification and higher magnification images of perfused brain slices, and two-photon microscopy live imaging allows for a multifaceted assessment of perfusion changes.
Authors and Affiliations
Xinxuan YANG, Hao TIAN, Jiahui ZHAO, Lina ZHENG, Liping LIU
Keywords
Related Articles
- EP ID EP750399
- DOI 10.3969/j.issn.1002-0152.2024.01.001
- Views 1
- Downloads 0
Stereotactic electroencephalography-guided electrical stimulation-induced smile and laughter: a report of two cases
The network of laughter/smile production and propagation in the brain is not yet fully understood. In this paper, we report two cases of medically refractory epilepsy patients with stereotactic EEG implantation, in which...
Advance in research on EEG technology in non-suicidal self-injurious behaviour and suicidal ideation in depression
Non-suicidal self-injury behavior and suicidal ideation are important risk factors for suicide attempts in patients with depression. At present, studies have found that there are obvious differences in electroencephalogr...
A case of Lambert-Eaton myasthenia syndrome with the onset of ataxia
Lambert-Eaton myasthenic syndrome (LEMS) is an immune-mediated disorder involving neuromuscular junctions, characterized by muscle weakness, autonomic dysfunction, and reduced tendon reflexes. We report a LEMS case prese...
Effect of injection volume on clinical efficacy after minimally invasive puncture and drainage in the treatment of moderate basal ganglia cerebral hemorrhage underlying controlling the dosage of drugs in the elderly
[Objective] To investigate the effect of injection volume on clinical efficacy after minimally invasive puncture and drainage in the treatment of moderate basal ganglia cerebral hemorrhage underlying controlling the dosa...
Advances in morphology-based image post-processing techniques in the diagnosis and management of focal cortical dysplasia
Focal cortical dysplasia (FCD) is one of the most common etiologies leading to drug-resistant epilepsy (DRE), and its neuroimaging features are an important part of clinical evaluation. Only some types of FCD can show ab...