ESTABLISHMENT OF CALLUS AND SUSPENSION CULTURE IN SESAMUM INDICUM L
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 4
Abstract
Objective: Sesame (Sesamum indicum L.) is one of the most important oilseed crops in the world. We intended to evaluate the effects of different explants, carbon sources and growth regulators on callus induction and cell suspension culture of two Iranian sesame cultivars (Yekta and Karaj1).Methods: The explants (hypocotyl and cotyledon) were cultured on Murashige and Skoog (MS) basal medium supplemented with 30 g/l sucrose, 8 g/l agar, different concentrations and combinations of NAA, 2, 4-D, BAP and Kin hormones. To produce cell suspension culture, callus was excised and transferred to liquid MS medium supplemented with different concentrations of carbon source (glucose and sucrose) and growth regulators of auxins and cytokinins. The cultures were maintained under both dark and light conditions at different rotation speeds of the gyratory shaker.Results: Callus produced from hypocotyl explants grew faster during 7 to 30 days of culture and after that stabilized at a lower growth rate. Maximum growth rate of suspension culture was achieved from friable green callus in MS liquid medium supplemented with 3 mg/l NAA and 0.6 mg/l BAP using hypocotyl callus.Conclusion: The optimized cell suspension culture can be used to elicit the production of secondary metabolites, scale-up mass production in bioreactors and gene transformation.Â
Authors and Affiliations
Maryam Heidarifar, Fatemeh Dehghan Nayeri
Keywords
Related Articles
- EP ID EP578879
- DOI -
- Views 99
- Downloads 0
DEVELOPMENT OF COSMOCEUTICAL PRODUCTS FROM NANO-SIZED ACTIVE COLOUR CONSTITUENTS OF RATANJOT, SEABUCKTHORN AND ANNATTO
Objective: The objective of this research was to develop the nano-particles of natural colours from Ratanjot root, Seabuckthorn pulp, and Annatto seeds and use them in cosmetic products. Multiple herbs were used to have...
IN VITRO ANTIOXIDANT CAPACITIES AND ANTIDIABETIC PROPERTIES OF PLUCHEA LEAVES AND GREEN TEA MIXTURES AT VARIOUS PROPORTIONS
Objective: This study was done to determine the effect of pluchea leaves and green tea mixtures at various proportions to antioxidant and antidiabetic properties.Methods: The research used a single factor randomized bloc...
CYTOTOXICITY EVALUATION OF TITANIUM AND ZINC OXIDE NANOPARTICLES ON HUMAN CELL LINES
Objective: In vitro cytotoxicity evaluation of titanium dioxide, 20 nm (TNP 20) and zinc oxide, 20 nm (ZNP 20) nanoparticles (NP) were tested on different types of human skin (HaCat), lung (A549), liver (Hep G2) and colo...
QUANTITATIVE STRUCTURE–PHARMACOKINETICS RELATIONSHIP FOR PLASMA PROTEIN BINDING OF NEUTRAL DRUGS
Objective: Plasma protein binding (PPB) of drugs is important pharmacokinetic (PK) phenomena controlling the free drug concentration in plasma and the overall PK and pharmacodynamic profile. Prediction of PPB at the very...
ANTIOXIDANT AND HEPATOPROTECTIVE EFFECTS OF JUSTICIA SPICIGERA ETHYL ACETATE FRACTION AND CHARACTERIZATION OF ITS ANTHOCYANIN CONTENT
Objective: The antioxidant and hepatoprotective activities of ethyl acetate (EA) fraction of the dried aerial part of Justicia spicigera were evaluated and the characterization of its anthocyanin content was done. Metho...