ESTABLISHMENT OF CALLUS AND SUSPENSION CULTURE IN SESAMUM INDICUM L

Abstract

Objective: Sesame (Sesamum indicum L.) is one of the most important oilseed crops in the world. We intended to evaluate the effects of different explants, carbon sources and growth regulators on callus induction and cell suspension culture of two Iranian sesame cultivars (Yekta and Karaj1).Methods: The explants (hypocotyl and cotyledon) were cultured on Murashige and Skoog (MS) basal medium supplemented with 30 g/l sucrose, 8 g/l agar, different concentrations and combinations of NAA, 2, 4-D, BAP and Kin hormones. To produce cell suspension culture, callus was excised and transferred to liquid MS medium supplemented with different concentrations of carbon source (glucose and sucrose) and growth regulators of auxins and cytokinins. The cultures were maintained under both dark and light conditions at different rotation speeds of the gyratory shaker.Results: Callus produced from hypocotyl explants grew faster during 7 to 30 days of culture and after that stabilized at a lower growth rate. Maximum growth rate of suspension culture was achieved from friable green callus in MS liquid medium supplemented with 3 mg/l NAA and 0.6 mg/l BAP using hypocotyl callus.Conclusion: The optimized cell suspension culture can be used to elicit the production of secondary metabolites, scale-up mass production in bioreactors and gene transformation. 

Authors and Affiliations

Maryam Heidarifar, Fatemeh Dehghan Nayeri

Keywords

Related Articles

FORMULATION AND EVALUATION OF LIQUISOLID COMPACTS OF LORNOXICAM

Objective: Objective of the present investigation was to enhance the solubility and dissolution rate of poorly water-soluble drug lornoxicam using liquisolid technique with comparative determination of in vitro release p...

COST ANALYSIS OF EMERGENCY VISITS DUE TO DRUG RELATED PROBLEMS

Objective: To identify patients coming to Emergency Medicine Department (EMD) with drug related problems, classify the DRPs and calculate the direct cost spent for treating them. Methods: This was a prospective observat...

A PROSPECTIVE STUDY: KNOWLEDGE ASSESSMENT AND PATIENT CARE OF DIABETIC FOOT ULCER PATIENTS IN TERTIARY CARE HOSPITAL

Objective: To assess the knowledge with a diabetic foot ulcer, to evaluate the severity and grade of diabetic foot ulcer, to study the self-care behaviour and medication adherence in a patient with diabetic foot ulcer an...

FISHBURNE’s METHOD AND THE CLASSICAL METHOD OF PHARMACOECONOMIC ANALYSIS IN THE EVALUATION OF ANTIBIOTIC TREATMENT OF ACUTE AND RECURRENT BRONCHITIS IN CHILDREN

Objective: This study was performed in order to estimate clinical effectiveness of antibiotic therapy in acute and recurrent obstructive bronchitis. For estimation the following was used: the Fishbern method of antibioti...

EFFECT OF SUGNIL, A TRADITIONAL ANTIDIABETIC HERBAL FORMULATION ON THE EXPRESSION OF TRANSCRIPTION FACTORS IN STREPTOZOTOCIN DIABETIC RATS.

Objective: A number of large randomized clinical trials demonstrated that lipid lowering successfully reduced cardiovascular events and death in patients with diabetes. Recent studies have reported that multiple transcri...

Download PDF file
  • EP ID EP578879
  • DOI -
  • Views 57
  • Downloads 0

How To Cite

Maryam Heidarifar, Fatemeh Dehghan Nayeri (2015). ESTABLISHMENT OF CALLUS AND SUSPENSION CULTURE IN SESAMUM INDICUM L. International Journal of Pharmacy and Pharmaceutical Sciences, 7(4), 255-258. https://europub.co.uk/articles/-A-578879