ESTABLISHMENT OF CELL SUSPENSION CULTURE FROM ONOSMA DASYTRICHUM SEED CALLUS CULTURE
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2011, Vol 2, Issue 10
Abstract
The initiation of callus production and establishment of cell suspension culture from Onosma dasytrichum are described in this study. In this study, using different plant growth media and various concentrations of sucrose and 2,4-D, the growth patterns of cell suspension cultures were examined. Murashige and Skoog medium was identified as best medium for cell suspension culture. Medium containing 2,4-D (0.2 mg/L) and 40 mg/L sucrose induced higher rates of cell division and produced more cell increase ratio for fresh weight (CIRFW) than other media which were examined.
Authors and Affiliations
SAMANE BAGERI| National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran,Faculty of Agricultural Sciences, Payame Noor University, Tehran, Iran, FOROUGH SANJARIAN*| National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran, KAMALEDIN HAGHBEEN| National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran, MOHAMMMAD ALI EBRAHIMI| Faculty of Agricultural Sciences, Payame Noor University, Tehran, Iran
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