ESTIMATION OF ANALYTICAL PARAMETERS OF TLC-METHOD FOR ANALYSIS OF DOCOSAHEXAENOIC ACID AS COMPOUND OF OMEGA-3 POLYUNSATURATED FATTY ACIDS
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2017, Vol 6, Issue 3
Abstract
The aim of current study was the estimation of the analytical parameters linearity, accuracy and precision of simple TLC-method for analysis of Docosahexaenoic acid, as a component of Omega-3 polyunsaturated fatty acids. The aim was to be proposed simple, easy and not expensive method. After the experiments with different mobile phases, was proved that the most appropriate mobile phase was: cyclohexane : ether : benzene = 8 : 4 : 1 v/v/v. The developed Silicagel G60F254 plates were dried on air and the plates were examined on UV-lamp at λ = 254 nm. The selectivity of the applied TLC-method was confirmed by the fact, that on chromatogram with placebo preparation did not exist spot with Rf, corresponding to RfDocosahexaenoic acid = 0.3. The linearity accordance between the concentration and spot area in concentration range: 2.10–3 g/ml ÷ 5.10–2 g/ml was proved by the regression equation: y = 44.11.x + 0.038.
Authors and Affiliations
Dobrina Doncheva Tsvetkova
Keywords
Related Articles
- EP ID EP617704
- DOI -
- Views 139
- Downloads 0
A REVIEW ON COMPUTER AIDED DRUG DESIGN IN DRUG DISCOVERY
Computer aided drug design (CADD) is an evolving cascade of research area encompassing many facets. Computer-aided drug design (CADD) is an exciting and diverse discipline where various aspects of applied and basic resea...
RISK FACTORS, CAUSES AND OUTCOME OF POSTPARTUM HEMORRHAGE IN SULAIMANIA MATERNITY TEACHING HOSPITAL
Postpartum hemorrhage (PPH) is still one of the leading causes of maternal morbidity and mortality. Blood loss in excess of 500ml after vaginal delivery or 1000 after C/S in the first 24 hours, is defined as primary PPH,...
A STUDY TO DETERMINE TOTAL PHENOLIC CONTENT OF OPUNTIA FICUS-INDICA EXTRACTS AND THEIR ACTIVITY AGAINST SOME PATHOGENIC FUNGI
This study was carried out to determine total phenolic content Of cactus plant extracts (Opuntia ficus-indica) and their activity against some pathogenic fungi. The results of analysis of total phenolic content of methan...
IN VIVO – IN VITRO CORRELATION – A STRATEGIC TOOL FOR DRUG DEVELOPMENT
Development and optimization of formulation is an integral part of manufacturing and marketing of any therapeutic agent, which is a time consuming and costly process. Correlation between In Vivo – In Vitro (IVIV) data is...
ANTIDIABETIC AND HYPOLIPIDEMIC EFFECT OF ASPARAGUS RACEMOSUS IN ALLOXAN INDUCED DIABETES IN RATS.
The Asparagus racemosus was evaluated for its antidiabetic activities and hypolipidemic activity against alloxan induced hyperglycemia in rats. Thirty rats were used divided into 2 groups, normal control: Composed from 1...