Estimation of Isolated Triterpenoid - Ursolic Acid in Verbena officinalis L. Aerial Parts Using TLC Densitometry
Journal Title: JOURNAL OF PHARMACEUTICAL TECHNOLOGY, RESEARCH AND MANAGEMENT - Year 2014, Vol 2, Issue 2
Abstract
Verbena officinalis L. (Vervain; family-Verbenaceae),a traditionally used and medicinally promising plant, has been reported to contain triterpenoids as major class of phytoconstituents. But no work has ever been carried out on V. officinalis to isolate major chemical constituent(s), and to standardize plant material on the basis of isolated constituent(s) using TLC densitometry.The chloroform extract of V. officinalisaerial parts was prepared by extracting properly identified plant in a Soxhlet apparatus.Preliminary phytochemical screening of chloroform extract showed presence of triterpenoids. Column chromatography of chloroform extract using solvent systems viz., hexane:chloroform, chloroform and chloroform:methanol in different concentrationsyielded five fractions (F1- F5). Preliminary phytochemical screening of various fractions of chloroform extract revealed presence of triterpenoids in F2 and F3 fractions. Therefore, these fractions were further subjected to column chromatography. White colored crystals were obtained in SF1 sub-fraction separated from F2and was designated as VOC-1. Structure of VOC-1 was elucidated by IR and NMRspectral studies and was characterized as ursolic acid. Further, ursolic acid was quantified in V. officinalisaerial parts by validated TLC densitometric method. The content of ursolic acid was found to be 0.1580% in V. officinalis aerial parts.The linearity range, correlation coefficient, intra-day precision, inter-day precision, LOD, LOQ and accuracy were found to be 300-1800 ng, 0.997, 1.2% CV, 1.6% CV, 40 ng/spot, 130 ng/spot and 98.27% respectively.
Authors and Affiliations
Jashanjot Kaur , Deepak Kumar , Reecha Madaan , Suresh Kumar
Keywords
Related Articles
- EP ID EP596688
- DOI 10.15415/jptrm.2014.22008
- Views 173
- Downloads 0
Formulation, Optimization and Evaluation of Sustained Release Microspheres using Taguchi Design
The aim of present study is to prepare microspheres of eudragit RL 100 loaded with Nefopam Hydrochloride by single emulsion solvent evaporation technique. Taguchi L9 orthogonal array design has been used to optimize the...
Microneedle Mediated Vaccine Delivery: A Comprehensive Review
Microneedles can be representative for paradigm shift of drug delivery from patient non-compliant parenteral injections to patient compliant drug delivery system, which can be utilized for administration of vaccines part...
Improvement of Learning and Memory of Mice by Plumbagin
Roots of Plumbago zeylanica have been reported to improve learning and memory of mice, but the effect of plumbagin on learning and memory of mice and the possible mechanisms for its effect on memory have not been explore...
An Update on Some Recent Solubility Enhancers as Pharmaceutical Excipients
At present the pharmaceutical academia and industries are focusing on the use of natural materials and resources for development of pharmaceutical product. Due to advances in drug delivery technology, currently, excipien...
Protective Role of Herbal Drugs in Diabetic Neuropathy: An Updated Review
Medicinal plants play a beneficial role in health care and are commonly used in preventing and testing diseases and specific ailments. The advantage associated with herbals plants are numerous and cannot be ignored as th...