Estimation of Proinflammatory Cytokines in Platelet Concentrates Prepared by Buffy Coat Method and Prestorage Leukofiltered/Non-Leukofiltered Platelet Rich Plasma Method – A Comparative Study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 2
Abstract
The platelet concentrates is one of the indispensable blood components transfused in practice. Platelet concentrates are prepared by Platelet Rich Plasma (PRP) method, Buffy Coat (BC) method and apheresis method. There was growing concern about White Blood Cells (WBCs) in blood components as WBCs were involved in pathogenesis of transfusion reactions. The reduction of WBCs in PCs prevented Febrile Non Hemolytic Transfusion Reaction (FNHTR), transmission of viruses and platelet alloimmunisation. Leukoreduction is also done to reduce the level of the cytokines released by the WBCs and thereby to reduce transfusion reactions. To compare and estimate the levels of Interleukin-6 (IL-6), Interleukin-8 (IL-8) and Tumor Necrosis Factor-α (TNF-α) in stored platelet concentrates prepared by Buffy Coat Method and Prestorage Leukofiltered/Non-Leukofiltered Platelet Rich Plasma Method at different storage time periods. During the study period of one and half years, proinflammatory cytokines IL-6, IL-8 and TNF-α were estimated on days 0, 3 and 5 of storage in 30 platelet concentrates each, prepared by Non-Leukofiltered Platelet Rich Plasma method (Non-LF PRP – PC), Buffy Coat method (BC – PC) and Leukofiltered Platelet Rich Plasma method (LF PRP – PC).These cytokines were estimated along with other parameters such as volume, platelet count and WBC count.In the present study, the WBC count in LF PRP-PCs and BC-PCs were significantly lower than Non-LF PRP- PCs. The difference in WBC count between BC-PCs and LF PRP-PCs was much less in comparison to the difference between Non-LF PRP-PCS and LF PRP-PCs. The level of proinflammatory cytokines IL-6, IL-8 and TNF-α on days 0, 3 and 5 of storage in Non-LF PRP-PCs showed a significant increase compared to BC-PCs and LF PRP-PCs. However, the difference between BC-PCs and LF PRP-PCs was considerably lower than the difference between Non-LF PRP-PCs and LF PRP -PCs. There was a statistically significant correlation between the WBC count in PCs and cytokine levels on different storage periods. Our study on estimation of the levels of Proinflammatory Cytokines suggests the usage of platelet concentrates prepared by Buffy Coat method to prevent FNHTR, the most frequent complication caused by increased levels of Cytokines. However, since alloimmunisation and platelet refractoriness cannot be prevented by Buffy Coat method, prestorage leukofiltration may be considered for selective cases.
Authors and Affiliations
R. Sreedevi, P. Arumugam, Swathandran Hamsavardhini
Keywords
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- EP ID EP464645
- DOI 10.9790/0853-1802021424.
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