Estimation of Total Phenols and Flavonoids in Selected Indian Traditional Plants
Journal Title: JOURNAL OF PHARMACEUTICAL TECHNOLOGY, RESEARCH AND MANAGEMENT - Year 2014, Vol 2, Issue 1
Abstract
Traditionally, aerial parts of Abies pindrow Royle (Himalayan Fir; Pinaceae); Abies webbiana Lindl. (Talispatra; Pinaceae); Cephalandra indica Naud. (Ivy Gourd; Cucurbitaceae) and roots of Calotropis gigantea (L.) Dryand (Giant Milkweed; Asclepiadaceae) have been used in the Indian systems of medicine for the treatment of various ailments. But no systematic phytochemical work has ever been carried out on these potential plants. Thus, it was planned to estimate total phenols and flavonoids content in methanol extract, ethyl acetate fraction (EAF) and remaining methanol extract (RME) of selected plants. Properly identified plants were defatted with petroleum ether, and then separately extracted in a Soxhlet apparatus with methanol. The methanol extract of each plant was partitioned by ethyl acetate solvent to obtain EAF of respective plant. The total phenols and flavonoids contents were estimated using standardized procedures. Quantitative determination of total phenols and total flavonoids was done using standard curve of gallic acid (linearity: 20 to 120 mg/ml; r2 = 0.995) and quercetin (linearity: 30 to 180 mg/ml; r2 = 0.997), respectively. EAF of selected plants contained higher content of phenols and flavonoids, where as lowest content was observed in RME of plants. The content of total phenols and flavonoids in selected plants were found to be in order of C. indica > A. webbiana > A. pindrow > C. gigantea. The available literature reveals that polyphenols have been reported to possess varied pharmacological activities. As selected Indian plants contain polyphenols as major class of phytoconstituents, it is suggested that these constituents may be responsible for their medicinal uses.
Authors and Affiliations
Deepak Kumar , Anupam Jamwal , Reecha Madaan , Suresh Kumar
Keywords
Related Articles
- EP ID EP596686
- DOI 10.15415/jptrm.2014.21006
- Views 148
- Downloads 0
Pulsatile Drug Delivery System – A Novel Approach for Time and Spatial Controlled Drug Delivery
The area of pharmaceutical research is broadened with the invention of new pharmaceutical drug delivery system. The traditional drug deliveries systems have not the way to treat or cure special disease or as well as the...
Antihepatotoxic Potential of Citrullus colocynthis Root Extract, Fractions and Isolated Compounds
Medicinal plants are considered to be effective and safe alternative treatment for liver toxicity. The article reveals the hepatoprotective activity of the ethanolic extract of the roots of the Citrullus colocynthis comm...
A Study of Prescribing Habits of Low Molecular Weight Heparin in Cardiovascular Disease in Tertiary Care Hospital
Background and Objective: Cardiovascular Diseases (CVDs) are the leading non-communicable diseases and also leading cause of morbidity and mortality worldwide. Low Molecular Weight Heparin (LMWHs) is frequen...
Toxicological Evaluation of Crude Alkaloid Fraction Isolated From Indian Folklore Plant Telosma Pallida (Roxb) wg Craib Root Using Probit Value Analysis
Present study first time reported the toxicological profile of Telosma pallida (TP) crude alkaloid fraction (CAF) isolated from the root of the climber plant by brine shrimp lethality test (BLT). Telosma pallida is a per...
Dissolution Enhancement of Domperidone Fast Disintegrating Tablet Using Modified Locust Bean Gum by Solid Dispersion Technique
Enhancement of dissolution characteristics of poorly soluble drug Domperidone by solid dispersion technique using modified locust bean gum (MLBG) and further conversion into tablet dosage form with fast dissolving charac...