Evaluación de la susceptibilidad de Enterococcus faecalis ATCC-29212 frente a medicamentos combinados con hidróxido de calcio
Journal Title: Revista Eugenio Espejo - Year 2021, Vol 15, Issue 1
Abstract
The high incidence of Enterococcus faecalis in dental procedures is a topic of interest for the health area. This bacterium resists various antimicrobials, and its proliferation increases due to its low susceptibility to substances of conventional use such as calcium hydroxide, becoming a of the main causes of root canal failure. The present investigation evaluated the in vitro susceptibility of Enterococcus faecalis strain ATCC-29212 to the combination of substances and antibiotics with calcium hydroxide. The agar diffusion technique was used, inoculating the microorganism in Petri dishes with Brain Heart agar (BHI), treated with filter paper disks impregnated with 1μl of each treatment in triplicate; T1 (Calcium Hydroxide + propylene glycol), T2 (Calcium Hydroxide + paramonochlorophenol), T3 (Calcium Hydroxide + ampicillin gentamicin + propylene glycol), T4 (Calcium Hydroxide + Physiological Saline Solution), Positive control (Hydroxide-based commercial medicine Calcium), incubated at 35 ° C for 24h, the halos formed around each disc were measured and compared with the Duraffourd scale and processed by one-factor ANOVA. A measure of the inhibition halo of 22.50 ± 3.3 mm was obtained with the T3 treatment, being extremely sensitive. It was concluded that this was the most effective in comparison with the rest of the in vitro tests of the rest of the investigated drugs.
Authors and Affiliations
Doménica Mishelly Rodríguez Robalino Centro de Salud Tipo B Guano, Ecuador https://orcid. org/00000002-0261-4909 Silvia Alexandra Reinoso Ortiz Universidad Nacional de Chimborazo, Ecuador https://orcid. org/00000002-8895-8947 Silvia Verónica Vallejo Lara Universidad Nacional de Chimborazo, Ecuador https://orcid. org/00000001-9857-4157 Paola Natali Paredes Chinizaca Universidad Nacional de Chimborazo, Ecuador https://orcid. org/0000-0003-0453-7019
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