Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2016, Vol 6, Issue 4
Abstract
Objectives: The aim of this study was to analyze the isolated cholera strains at outbreak 2013 for studying the their similarity and compare their homology in order to find out the route of infection either emerge from abroad or reemerge from inside native strains. Methods: All diagnosed V. cholerae isolates were entered to the study after re-identification at referral laboratory of Health Ministry based on standard procedures. These specimens were examined for specific serogroups by O1 poly-valent and Ogawa/Inaba nonspecific antisera and tested by MIC Test Strip Method against Ciprofloxacin, Nalidixic Acid, Cefixime, Ampicillin, Tetracycline, Trimethoprim-Sulfamethoxazole, and Erythromycin. Results: A total of 257 clinical Vibrio cholerae was isolated in an outbreak of Iran at 2013. The dominant causative type was Inaba. In Antibiotic susceptibility test isolates were 100% resistant to all except Erythromycin that just 23% of strains were sensitive. Homology of isolates was investigated through genotyping by PFGE method and their clonality was compared with previous isolated Iranian native strain. Overall 92% of analyzed strains showed a homolog pattern. These strains were located in 8 clusters. Although isolated strains at 2011 had 80 % homology with recent isolates, located in complete distinct cluster than all strains isolated at 2013. PFGE analysis revealed no dissimilarity between those stains resistant and sensitive to Erythromycin. Conclusion: This study confirmed that isolated Inaba strains at 2013 had different clonality pattern in PFGE than previously identified, suggested have foreign route from the neighboring countries. J Microbiol Infect Dis 2016;6(4): 184-189
Authors and Affiliations
Massoud Hajia, Alireza Dolatyar, Marjan Rahnamaye Farzami, Mohsen Imani, Roghieh Saburian, Mohamad Rahbar
Keywords
Related Articles
- EP ID EP412734
- DOI 10.5799/jmid.328934
- Views 85
- Downloads 0
Güney Nijerya’da hamile kadınlar arasında cytomegalovirus seroprevalansı ve risk faktörleri
Amaç: Sağırlık ve zekâ geriliğine de yol açan vertikal cytomegalovirus (CMV) enfeksiyonu dünya’da önlenebilir konjenital enfeksiyonlarının en sık sebebidir. Halen dünya’da rutin antenatal tarama konusunda bir fikir birl...
Pulmonary Embolism an Uncommon First Presenting Symptom of an Undiagnosed Case of HIV
A 32 years old male who presented to our emergency department with shortness of breath, cough and weight loss, all started less than a month time and later diagnosed with HIV infection with very high viral load and low C...
Hastane personeli, yatan hastalar ve toplumda nazal MRSA taşıyıcılığı ve klonal bağlantı
Amaç: Sağlık çalışanları, hospitalize hastalar ve sağlıklı gönüllülerde nazal metisilin-dirençli Staphylococcus aureus (MRSA) kolonizasyonu ve klonal dağılımı pulsed field gel electrophoresis (PFGE) ile araştırıldı. Yö...
Febril nötropenik kanser hastalarında serum amiloid-A and C-reaktif protein CRP’nin enfeksiyon için öngörü değeri
Amaç:Febril nötropeni (FEN)si olan hastalarda serum amyloid A (SAA) ve C-reaktif protein (CRP)’in enfeksiyonun erkentanısı ve mortaliteyi öngörmede değerini incelemek Yöntemler:Antibiyoterapi sırasında hastaların serum...
Persistent colonization of Helicobacter pylori in human gut induces gastroduodenal diseases
Helicobacter pylori are gut bacteria colonize in the epithelial cell lining of the stomach and persist there for long duration. Around two-thirds of the world’s populations are infected with H. pylori and cause more th...