Evaluation of a Chromogenic Medium for the Detection of ESBL with Comparison to Double Disk Synergy Test
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2017, Vol 21, Issue 12
Abstract
Background: Extended Spectrum Beta Lactamase (ESBL) producing bacterial strains are the major causes of nosocomial and community-acquired infections worldwide. The aim of the study was to evaluate the effectiveness of Brilliance ESBL Agar (BEA) (a chromogenic culture medium) for the detection of ESBL in comparison with Double Disc Synergy Test (DDST) and confirm results from both methods by Single-plex Polymerase Chain Reaction (PCR) as gold standard. Materials and Methods: A total of 75 clinical isolates of Escherichia coli were screened for ESBL production using BEA & DDST from various clinical specimens. The antibiotic susceptibility testing was done by the Kirby-Bauer disc diffusion method using Cefotaxime (30 µg) and Ceftazidime (30 µg) discs on Mueller Hinton agar. ESBL producing strains were detected phenotypically by DDST and BEA at 24 h and 48 h, respectively. Isolates screened by both methods were confirmed using PCR for the detection of blaSHV, blaTEM, blaCTX-M genes. Results: The prevalence of ESBL was 61%. The sensitivity and specificity of DDST at 24 h and 48hours incubation time was 91.3% and 89.5%, respectively. BEA showed an increase in sensitivity and specificity at 48 h with 97.8% and 98.0%, respectively. All ESBL producing strains detected by phenotypic tests were also found harboring ESBL genes (blaSHV, blaTEM, blaCTX-M) by PCR. Conclusion: The use of BEA in the screening of ESBL production was found to give much better results than DDST and can be used where PCR cannot be performed.
Authors and Affiliations
C. C. Ezeanya, N. R. Agbakoba, C. E. Ejike, S. I. Okwelogu
Keywords
Related Articles
- EP ID EP311848
- DOI 10.9734/BJMMR/2017/33259
- Views 78
- Downloads 0
Factors Associated with Exclusive Breastfeeding Practices among Mothers in Syria: A Cross-sectional Study
Background: Exclusive breastfeeding is the situation where the infant has received only breast milk from his/her mother for the first 6 months after birth. Research had found that factors associated with exclusive breast...
Diagnostic Value of Coronary Artery Calcium Score for Cardiovascular Disease in African Americans: The Jackson Heart Study
Background: The role of coronary artery calcium (CAC) as a screening tool for cardiovascular disease (CVD) risk in African Americans (AAs) is unclear. We compared the diagnostic accuracy for CVD prevalence using the CAC...
Surgical Management of Haemangiolymphangioma - An Infrequent Tumor in Oral Cavity: A Case Report and a Review
Hemangiomas are group of vascular anomalies which are not present at birth. They generally manifest within 3-4 weeks of life, consists of a rapid proliferative phase, stable plateau phase and slow involuting phase. Heman...
Aggressive Angiomyxoma of the Vulva: Libyan Female Case Report
Aims and Background: Aggressive angiomyxoma is rare tumor of pelvic and perineal organs, occurring usually in women of reproductive age, and carrying a high tendency to local infiltration and relapse. Most literature abo...
Vitamin D Levels in Men with Metabolic Syndrome with or Without Hypogonadism
Aims: To clarify the relation of metabolic syndrome (MS) with or without late-onset hypogonadism (LOH) and vitamin D deficiency in men. Study Design: Consecutive male patients with MS were additionally evaluated for LOH...