Evaluation of a Chromogenic Medium for the Detection of ESBL with Comparison to Double Disk Synergy Test
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2017, Vol 21, Issue 12
Abstract
Background: Extended Spectrum Beta Lactamase (ESBL) producing bacterial strains are the major causes of nosocomial and community-acquired infections worldwide. The aim of the study was to evaluate the effectiveness of Brilliance ESBL Agar (BEA) (a chromogenic culture medium) for the detection of ESBL in comparison with Double Disc Synergy Test (DDST) and confirm results from both methods by Single-plex Polymerase Chain Reaction (PCR) as gold standard. Materials and Methods: A total of 75 clinical isolates of Escherichia coli were screened for ESBL production using BEA & DDST from various clinical specimens. The antibiotic susceptibility testing was done by the Kirby-Bauer disc diffusion method using Cefotaxime (30 µg) and Ceftazidime (30 µg) discs on Mueller Hinton agar. ESBL producing strains were detected phenotypically by DDST and BEA at 24 h and 48 h, respectively. Isolates screened by both methods were confirmed using PCR for the detection of blaSHV, blaTEM, blaCTX-M genes. Results: The prevalence of ESBL was 61%. The sensitivity and specificity of DDST at 24 h and 48hours incubation time was 91.3% and 89.5%, respectively. BEA showed an increase in sensitivity and specificity at 48 h with 97.8% and 98.0%, respectively. All ESBL producing strains detected by phenotypic tests were also found harboring ESBL genes (blaSHV, blaTEM, blaCTX-M) by PCR. Conclusion: The use of BEA in the screening of ESBL production was found to give much better results than DDST and can be used where PCR cannot be performed.
Authors and Affiliations
C. C. Ezeanya, N. R. Agbakoba, C. E. Ejike, S. I. Okwelogu
Keywords
Related Articles
- EP ID EP311848
- DOI 10.9734/BJMMR/2017/33259
- Views 70
- Downloads 0
Association of the Lipoprotein Lipase S447X and Neuropeptide Y Leu7Pro Genetic Polymorphisms with the Lipid Profiles of Individuals with and without Evidence of Coronary Artery Disease
Background: Coronary artery disease (CAD) is a multifactorial disease whose etiogenesis involves a number of environmental, genetic and lifestyle-related factors. Genetic polymorphisms are noteworthy among these factors...
Antinociceptive Effect of Ethanolic Extract of Hybanthus enneaspermus Leaf in Rats
Aims: To test the hypothesis that Hybanthus enneaspermus leaf has an antinociceptive effect. Methodology: Seventy-two male rats were randomly divided in a blinded fashion into 4 groups each for the tail immersion test (n...
Preliminary Study: Neurobehavioural Effects of Nauclea latifolia and Emilia sonchifolia in Mice Infected with Plasmodium berghei berghei
This study was designed to examine the effects of ethanolic leaf extracts of Nauclea latifolia and Emilia sonchifolia on anxiety, fear and locomotion in mice infected with plasmodium berghei berghei. Thirty male Swiss al...
Role of Anxiety on Patient Intolerance during Bronchoscopy
Aims: There are no current data about the adverse effects of anxiety on patient intolerance during bronchoscopy.The aim of our study was to assess the correlation between anxiety and patient intolerance during bronchosco...
Idiopathic Necrotizing Scleritis, Anterior Uveitis, and Localized Retinal Detachment
Aim: To present clinical picture and treatment of anterior idiopathic necrotizing scleritis. Methods: Clinical and laboratory examination; B-scan ultrasound; tissue biopsy and histological analysis, were performed. Resul...