Evaluation of a petit tissue microarray in a tertiary care histopathology laboratory– a prospective study
Journal Title: Pathology Update: Tropical Journal of Pathology and Microbiology - Year 2018, Vol 4, Issue 4
Abstract
Background: With the advancements in pathology for diagnosis of tumors, there is a need for technologies which provide test results with a short turn-around time. Also, with the increasing incidence of early diagnosis of tumor, detection of prognostic markers becomes a necessity. Tissue Micro-Array is one such technology in which tumor diagnosis, tumor markers and prognostic markers could be studied with limited tissue sample at a low cost. In this study, we evaluated the feasibility of a petit Tissue Microarray for immunohistochemical profiling of breast carcinomas. Materials and Methods: Tissue cores were obtained from random tissues which included placenta, breast tissue and lymphnode and endometrium using skin punch biopsy needle of bore 2 mm. These were done for standardizing the procedure of a miniature tissue microarray. Further, tissue cores obtained from carcinoma breast tissue by two different methods were used for constructing a microarray block. Sections of 4 micron thickness were taken and stained with hematoxylin and eosin stains. If satisfactory tumor tissue is present in this constructed block, then sections were taken for immunohistochemistry staining with ER, PR and HER2. Results: Directly constructed tissue blocks had better preservation of tumor tissue morphology compared to the blocks constructed from donor blocks. Also, directly constructed tissue blocks had the advantage of not mutilating the donor block which could be still used for further studies and reference. Immunohistochemistry revealed similar results as obtained during the routine histopathological sections. Also, the cost of the reagents used for immunohistochemistry was reduced by 200% as compared to the routine immunohistochemical staining procedure. Conclusion: A petit Tissue microarray is definitely possible in a tertiary care histopathology laboratory and can be utilized for immunohistochemical studies with multiple markers.
Authors and Affiliations
Priavadhana Rajan Prasaad, Srilakshmi Priya V, Bheema Rao G. , B. O. Parijatham, Lakshmi Prabha V
Keywords
Related Articles
- EP ID EP505904
- DOI 10.17511/ jopm. 2018.i4.10
- Views 123
- Downloads 0
Rapid method of cytology diagnosis by supravital staining in FNAC of various tissue and organs
Background: Supravital staining is the staining of living tissue removed from the body, but before cessation of the chemical life of the cells. Objective: The present study has been undertaken with the aim of to assess a...
Pathological and Microbiological Analysis of Cerebrospinal Fluid in Bacterial Meningitis
Background: Meningitis is a life threatening infection with incidences increasing in adults and children. CSF analysis is a key tool to diagnose and differentiate between bacterial, viral, tubercular and fungal meningiti...
Howell’s cytological grading of breast carcinomas - comparison with histological grading and its association with lymph node metastasis
Background: Determining histological grade of breast carcinomas before mastectomy is necessary to decide about neo-adjuvant chemotherapy. Grade obtained from fine needle aspiration cytology samples will help in such situ...
Serological profile of acute undifferentiated fever cases attending a tertiary care hospital: emergence of scrub typhus in Telangana State
Introduction: Present study was undertaken to elucidate aetiology of acute undifferentiated fevers and prevalence of scrub typhus among patients admitted in our hospital. Materials & Methods: 100 consecutive serum sample...
Liver autopsy study – incidental pathological findings
Background: Liver is the site of many diseases, many of which become symptomatic while some are diagnosed only on autopsy. Involvement of liver is secondary to cardiac, metabolic, social problem like alcoholism, drug tox...