Evaluation of anti A60 IGM for the diagnosis of tuberculosis with elisa method
Journal Title: Pakistan Journal of Pharmacology - Year 2011, Vol 28, Issue 2
Abstract
Evaluation of Anti Tuberculous IgM antibodies for rapid diagnosis of both pulmonary and extra-pulmonary tuberculosis. ELISA assay based on mycobacterial antigen A-60 (Anda Biologicals, France) was used on the sera obtained from 69 cases of Tuberculosis and 136 controls in the population of Karachi, Pakistan. Of 136 controls only 21.3 % were positive for IgM antibodies and showing 78.7% specificity. A very high sensitivity 72.2% was seen in sputum positive active pulmonary tuberculosis. Relatively low 56.2% sero positivity was seen in cases of sputum negative active pulmonary tuberculosis compared to those of sputum positive active pulmonary tuberculosis. In cases of extra pulmonary tuberculosis 57.8 % sensitivity was observed. In cases of healed tuberculosis only 18.7% were found positive for IgM. The estimation value of IgM against A-60 for the diagnosis of tuberculosis is proved from our data. Considering all the cases of active tuberculosis and the controls the global sensitivity of 62.2% and specificity of 78.7% was found when IgM estimation was taken into account.
Authors and Affiliations
Syed Ahmed, Saleem Kharal, Muhammad Shahzad
Keywords
Related Articles
- EP ID EP85999
- DOI -
- Views 153
- Downloads 0
To evaluate the feasibility of treating prehypertension with an angiotension receptor blocker candesartan cilexetil
Prehypertension is considered a precursor of stage 1 hypertension and a predictor of excessive cardiovascular risk. The trial of preventing hypertension (TROPHY) was an investigator-initiated study to examine whether ear...
Alendronate a promising / multi aspect drug in the management of ostoeoporosis
Recently, osteoporosis is regarded as a major health concern due to increase in its incidence, associated morbidities and mortalities. Among antiresorptives, alendronate which is a bisphosphonate is FDA (Food and Drug Ad...
Role of leukotriene receptor antagonist in acute severe attack of bronchial asthma in comparison with conventional therapy
This study was designed to determine the role of leukotriene receptor antagonist in acute severe attack of bronchial asthma in comparison with conventional therapy. Asthma is a chronic inflammatory lung disease in both t...
Comparative study of azelastine and verapamil in the modification of ovalbumin sensitized lung parenchymal tissues of guinea pigs in vitro
Pharmacological prevention of antigen-antibody mediated reaction can be accomplished, at least in principle either to prophylaxis achieved by inhibiting the release of chemical mediators and/or blocking the tissue recept...
Evaluation of anti A60 IGM for the diagnosis of tuberculosis with elisa method
Evaluation of Anti Tuberculous IgM antibodies for rapid diagnosis of both pulmonary and extra-pulmonary tuberculosis. ELISA assay based on mycobacterial antigen A-60 (Anda Biologicals, France) was used on the sera obtain...