Evaluation of anti-hemolytic activity of green tea aqueous extracts by in vitro method
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2017, Vol 6, Issue 2
Abstract
Hemolysis is a disorder of destruction of RBC cells in our body; chloroquine is the drug that regularly preferred to anti-malarial activity. This current study observes the destruction of RBC cells by chloroquine when treated with malarial disease and control of RBC lysis with addition of green tea extract along with chloroquine. Green tea leaves consist of phenolic compounds like flavonoids and catechin. Due to the presence of flavonoids and catechin which reduce the hemolysis of RBC by oxidative stress. This current study deals with concentration of hemolysis when induced with chloroquine and compared with aqueous extract of green tea with induced chloroquine and RBC suspension. This was clearly done by using the separation of the serum from plasma and induced the chloroquine to RBC suspension then the Heme generated was assayed and also absorbance was seen in UV visible photometry by following concentrations [high (2mg/ml) and low (1mg/ml)] of chloroquine. This same was repeated with addition of aqueous extraction of green tea by same concentration [high (1mg/ml) and low (2mg/ml)]. And observed that hemolysis taken place was 38.27% to 15.3%. (at low concentrations of chloroquine and green tea extract (1 mg/ml)). And also 55.56% to 26.17% (at high concentrations of chloroquine and green tea extract (2mg/ml)).
Authors and Affiliations
Mr. K. P. Raghava Krishna
Keywords
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