EVALUATION OF ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF FENUGREEK (TRIGONELLA FOENUM-GRAECUM) EXTRACTS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 4
Abstract
Objective: The present investigation was performed to evaluate the antimicrobial potential of Trigonella foenum-graecum leaves extracts and to elucidate the presence of phytochemicals responsible for its biological activity.Methods: The plant extracts were prepared by sequential cold maceration method by using four solvents viz., hexane, ethyl acetate, methanol and distilled water. Antimicrobial activity of extracts was carried out by agar well diffusion method against four bacteria and five fungi. Minimum inhibitory concentration (MIC) of different extracts was determined using the broth dilution method. Thin layer chromatography (TLC), TLC bioautography and phytochemicals analysis were also performed.Results: The antibacterial activity of T. foenum-graecum leaves extracts was found maximum on Serratia marcescens with a zone of inhibition (ZOI) of 12.33±0.57 mm by aqueous extract followed by inhibition of Bacillus cereus (ZOI = 11.50±0.50 mm) by the methanol extract. The range of MIC of different extracts recorded was 6.25 to 25 mg/ml. Results of the antifungal activity showed that methanol extract showed a maximum zone of inhibition against Trichoderma viridae (ZOI = 14.5±0.5 mm) followed by ethyl acetate extract (ZOI = 12.0±1.0 mm). The MIC value for methanol extract against T. viridae was 6.25 mg/ml. The result of TLC bioautography revealed that compounds eluted at Rf 0.58 and Rf 0.67 exhibited strong while compounds eluted at Rf 0.31 and Rf 0.37 showed moderate antibacterial activity against S. marcescens. Phytochemicals analysis indicated that methanol and aqueous extracts contain more phytochemicals as compared to hexane and ethyl acetate extracts.Conclusion: The results obtained in this study clearly indicate that T. foenum-graecum leaves extracts to have a significant potential to be used as an antimicrobial agent due to the presence of different phytochemicals.Keywords: Antimicrobial activity, Phytochemicals analysis, TLC bioautography, Minimum Inhibitory Concentration (MIC), Trigonella foenum-graecumÂ
Authors and Affiliations
Darshan Dharajiya, Hitesh Jasani, Tarun Khatrani, Manthan Kapuria, Karen Pachchigar, Payal Patel
Keywords
Related Articles
- EP ID EP577719
- DOI -
- Views 53
- Downloads 0
ANTIOXIDANT, FREE RADICAL SCAVENGING ACTIVITY AND GC-MS STUDIES ON PEDILANTHUS TITHYMALOIDES (L.) POIT
Objective: To evaluate the methanolic extract of the leaves of Pedilanthus tithymaloides for total phenol, total flavonoid, total antioxidant and free radical scavenging ability and detect the phytoconstituents using GC-...
COMPARATIVE STUDY OF REVERSE WET GRANULATION WITH CONVENTIONAL WET GRANULATION IN SOLUBILITY ENHANCEMENT OF SIMVASTATIN
Objectives: Simvastatin is a cholesterol- lowering agent widely used in hypercholesterolemia. It belongs to BCS class II drugs having low solubility and high permeability. Simvastatin is practically insoluble in water an...
EVALUATION OF RENAL EPITHELIAL CELL PROTEIN UNDER STRESS CONDITION
Objective: Proteins are an important component of cells which are involved in various cellular functions. Different kind of stressing conditions has different responses in the components of the protein synthesis system....
DRUG UTILIZATION PATTERN IN CHRONIC OBSTRUCTIVE PULMONARY DISEASE INPATIENTS AT A TERTIARY CARE HOSPITAL
Objective: Drug utilization studies provide useful insights into the current prescribing practices. In view of this, the present study was designed to establish the drug utilization pattern in hospitalized chronic obstru...
EFFECT OF CHLOROPHYLLIN, AN SEMI-SYNTHETIC CHLOROPHYLL MOLECULE ON HYPERGLYCEMIA AND HYPERLIPIDEMIA IN STREPTOZOTOCIN INDUCED DIABETIC MICE
Objective: The aim of the present study was to determine the effect of chlorophyllin (CHL) on hyperglycemia and hyperlipidemia in streptozotocin (STZ) induced diabetic mice.Methods: Diabetes was induced in mice by admini...