Evaluation of Antimicrobial Activity, Phytochemical Screening and Gas Chromatography-Mass/ Spectrophotometric Profile of Essential Oil from Persea americana & Citrus sinensis
Journal Title: Asian Journal of Medicine and Health - Year 2017, Vol 5, Issue 1
Abstract
This study aims to determine the phytochemical characteristics, structural determination of bioactive component from the leaf extract using GCMs and experimentally investigation of the antibacterial activity of essential oil from Persea americana and Citrus sinensis leaf against seven bacteria (Gram-positive and Gram-negative) which are Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, Klebsiella pneumonia, Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa known to be pathogenic to human causing high mortality among human. This study involves the extraction of essential oils from air- dried leaves of medicinal aromatic plant (Persea americana & Citrus sinensis) using soxhlet method with n- hexane as solvent. Antibacterial activity was carried out using agar well diffusion technique. Both plants essential oil shows high antibacterial property. The antibacterial sensitivity test revealed Escherichia coli having the widest zone of inhibition (20.0 mm) with Citrus sinensis essential oil at 100 mg/ml while the least zone of inhibition (5.0 mm) was recorded for Staphylococcus aureus at 12.5 mg/ml with essential oil of Persea americana however, Pseudomonas aeruginosa was found to be resistant to the oil. Phytochemical analysis of the plants shows the presence of active components such as Cardiac glycoside, Steroid, Anthraquinone, Flavonoid, Phenol, Alkaloid and reducing sugars. The presence of these components enhances the effectiveness of plants essential oil in the treatment of various diseases and also helps to act as an antibacterial agent. Persea americana essential oil was further analyzed by gas chromatography–mass spectroscopy (GC/MS), the main constituents were Squalene (45.94%), vitamin E (12.50%), Diisooctyl phthalate (7.45%) and 12-Methyl-E,E-2,13-octadecadien-1-ol (7.20%). These components aid the antibacterial activities of Persea americana essential oil.
Authors and Affiliations
Oludare Temitope Osuntokun, Peace O. Ukwuoma, A. M. Yusuf- Babatunde
Keywords
Related Articles
- EP ID EP336205
- DOI 10.9734/AJMAH/2017/33766
- Views 58
- Downloads 0
In vitro Impact Assessment of Aqueous Extract of Sida cordifolia Linn. Upon Rat Spermatozoa Parameters
Sida cordifolia Linn. finds frequent mention in Ayurvedic System of Medicine for many therapeutic properties including beneficial effect upon male reproductive processes. Aim of present in vitro study was to evaluate imp...
Helicobacter pylori Containing More Phosphorylation Sites of the CagA Protein Induces Greater Reduction of Gastric Mucins
Aims: H. pylori infection is associated with gastritis, pre cancerous lesions, gastric cancer and changes in the protective layer of mucus. However, to our knowledge, there are no studies in humans or experimental models...
Seroprevalence of Hepatitis B and Listeriosis Co-Infection among Patients Attending Sickbay Hospital, Ahmadu Bello University, Zaria, Kaduna, Nigeria
Aims: A cross-sectional research study on the seroprevalence of hepatitis B and listeriosis co-infection among patients attending Sickbay Hospital, Ahmadu Bello University, Zaria was carried out, with view to characteriz...
Prognostic Value of Elevated Cardiac Troponin I Levels in Pre-dialysis Chronic Kidney Disease Patients without Cardiac Symptoms
Introductory Concepts: Elevation of cardiac troponin I may be a marker of increased risk of cardiovascular outcomes such as left ventricular hypertrophy, congestive heart failure, ischemic heart disease and reduced survi...
Pattern of Ultrasound Request at the University of Port Harcourt Teaching Hospital, Nigeria
Aim: The aim of the study is to evaluate the pattern of ultrasound request in our tertiary health institution in order to make adequate recommendation on management and financing of imaging facilities in a hospital with...