Helicobacter pylori Containing More Phosphorylation Sites of the CagA Protein Induces Greater Reduction of Gastric Mucins
Journal Title: Asian Journal of Medicine and Health - Year 2017, Vol 5, Issue 1
Abstract
Aims: H. pylori infection is associated with gastritis, pre cancerous lesions, gastric cancer and changes in the protective layer of mucus. However, to our knowledge, there are no studies in humans or experimental models that relate the infection with H. pylori strains containing one or three phosphorylation sites of the CagA protein with the mucins of the protective layer of mucus. The aim of this study was to research the effects of infection with H. pylori strains containing one or three phosphorylation sites of CagA protein on the MUC1 and MUC5AC mucins of Mongolian gerbils (Meriones unguiculatus) gastric mucosa. Methods: Mongolian gerbils were inoculated with H. pylori isolates containing one or three EPIYA C phosphorylation sites. The control group consisted of uninfected animals receiving only Brucella broth. Gastric mucosa fragments were assessed by immunohistochemistry using rabbit monoclonal antibodies against MUC1 and MUC5AC. The fragments were then analyzed through a digital morphometric method. Results: From the control group of non-infected animals (CTRL) to the Cag A positive locci with 1 EPYIA C group (CagA1Ep) and Cag A positive locci with 3 EPYIA C group (CagA3EP) there was a progressive and significant reduction in the expression of both MUC1 (p=0.003) and MUC5AC (p=0.003) after 45 days of infection. This reduction was more prominent in the CagA3Ep group. Similar results were obtained six months post-infection, but the reduction in the MUC5AC (p=0.001) and MUC1 (p=0.001) expression was more intense. Conclusion: H. pylori strains containing more phosphorylation sites of the CagA protein are able to reduce with greater intensity the expression of MUC1 and MUC5AC.
Authors and Affiliations
Moacir Ferreira Júnior, Sérgio de Assis Batista, Rafael Calvão Barbuto, Adriana Dias Gomes, Dulciene Maria Magalhães Queiroz, Ivana Duval Araújo, Marcelo Vidigal Caliari
Keywords
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- EP ID EP336277
- DOI 10.9734/AJMAH/2017/34618
- Views 77
- Downloads 0
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