Evaluation of bond strength of human dentin subjected to different forms of storage
Journal Title: RSBO - Year 2010, Vol 7, Issue 3
Abstract
Introduction and objective: Human teeth are frequently used in laboratory and research activities. The aim of this study was to evaluate the bond strength of human dentin subjected to different forms of storage. Material and methods: Fifteen intact third molars were selected and divided into three experimental groups (n = 5): 1) teeth sterilized by autoclave for 15 minutes (121°C) and frozen in distilled water for 60 days; 2) teeth immersed in 2% glutaraldehyde solution for 24 hours and frozen in distilled water for 60 days; 3) only frozen in distilled water for 60 days (control). Samples were prepared with a diamond disk under cooling until obtaining flat surfaces of middle dentin, which were exposed by wet abrasion with 600-grit SiC paper in order to create smear layer prior to hybridization. The conventional adhesive system Adper Single Bond 2 and the composite Filtek Z250 (3M Espe) were used according to manufacturer’s instructions. Tygon tubing molds were placed on each sample and its internal volume was filled with the composite. After photoactivation, the molds were removed and the specimens were exposed and stored in distilled water at 37°C for 24 hours before microshear bond strength test. Results: The bond strength was calculated in MPa±SD, and data were statistically analyzed by Anova and Tukey test at 5% significance level. Data showed that the control group presented bond strength statistically higher than the other experimental groups. Conclusion: The autoclaving and immersion in glutaraldehyde solution reduced significantly the bond strength in human dentin when compared to frozen teeth in distilled water.
Authors and Affiliations
Ana Jacob, Mariana Fraga, Rubens Garcia, Luciano Madeira
Keywords
Related Articles
- EP ID EP144793
- DOI -
- Views 162
- Downloads 0
Comparison of the sealing ability of different types of glass ionomer cements in temporary restoration
Introduction: Glass ionomer cement has been used for temporary restoration; however, several formulations are available. Objective: The aim of this study was compare the linear leakage of different types of glass i...
Regeneração tecidual Guiada sem Tratamento de Defeitos de furca classe II
Introdução: Várias técnicas cirúrgicas têm sido avaliados na tentativa de regeneração de defeitos de furca. Objetivo e revisão da literatura: Entre as técnicas regenerativas, a regeneração tecidual guiada (RTG) tem sido...
Use of dental records for human identification
Introduction and objective: The present text consists in an analysis about the use of dental records to the identification of charred corpses,putrefied bodies or skeletal remains, based on a real application in a forensi...
Ankyloglossia: case report
Introduction: The lingual frenulum is an anatomic structure that plays an important role in the act of suction, speech and feeding. A short and adhered lingual frenulum obstructs the tongue movement. This can impair the...
Apicoectomy after conventional endodontic treatment failure: case report
Introduction: Paraendodontic surgery is a safe and adequate alternative when teeth are not responding to conventional treatment and endodontic re-treatment. It must only be applied in specific situations. Endodonti...