EVALUATION OF CELL DEATH MECHANISMS INDUCED BY HYPERICIN AND ITS DERIVATIVE IN COLORECTAL CARCINOMA MODELS
Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2019, Vol 6, Issue 10
Abstract
Colorectal cancer (CRC) is the third most malignancy and the fourth human killer. Photodynamic therapy (PDT) has emerged as a promising alternative to conventional cancer therapies. This study aimed to investigate the ability of Hypericin (HY) and its derivative (HAHY) as synthetic photosensitizers (PSs) to induce tumoricidal effect in HCT-116 carcinoma models. Sixty balb/c male mice were transplanted with 5×105 HCT-116 cells to induce carcinoma bearing mice model. The experimental animals were then divided into five groups; Group I (untreated), Group II (HY-PDT), Group III (HAHY-PDT), Group IV (HY-PDT+EGCG) and Group V (FOLFOX). Blood, liver and tumor mass samples were taken. Also, biochemical studies and MTT assay were performed. HY and HAHY-PDT had a significant cytotoxic effect on human HCT-116 cells. Also, a significant upregulation in gene expression of P53 and CYT-c in concentration dose dependent manner. Furthermore, a significant increase in IL-6 gene expression in group (2) higher than that in group (3).Whereas it decreased in group (5), and showed insignificant difference in group (4) .Also, results showed decrease in VEGF gene expression in both group (3) and (4), while it showed significant increase in group (2) and slight increase in group (5). In conclusion, these PSs possess a strong cytotoxic and apoptotic effect on human HCT-116 cells. Using of EGCG along with HY-PDT improves the outcome. Also, HAHY-PDT can be used as an alternative to HY-PDT, where it showed tumor shutdown without metastasis besides its antitumor immunity.
Authors and Affiliations
Hala M. Ali
Keywords
Related Articles
- EP ID EP674557
- DOI -
- Views 183
- Downloads 0
KNOWLEDGE ABOUT RISK OF TANNING ON INCIDENCE OF SKIN CANCER AMONG SAUDI UNIVERSITY STUDENTS
Introduction: The evaluation of the knowledge gaps and attitudes towards protection measures against unnecessary sun exposure, including tanning, can act as guidance for planning of the prevention programs. This study ai...
AN APPROACH TO DIAGNOSIS OF T-CELL AND NK-CELL NON - HODGKIN LYMPHOMAS BY FLOW CYTOMETRIC IMMUNOPHENOTYPING ON FINE NEEDLE ASPIRATE OF LYMPH NODE
Introduction: Lymphoid Neoplasms are defined as clonal tumors of mature and immature B cells, T cells or natural killer (NK) cells at various stages of differentiation. Because NK cells are closely related and share some...
COMMON HEALTH COMPLAINT REPORTED BY STUDENTS ACCORDING TO SPECIALIZATION AND GENDER REGARDING SELF-MEDICATION AT AJMAN UNIVERSITY
Background: Self-medication is the selection and use of non-prescription medicines by individuals’ own initiatives to treat self-recognized illnesses or symptoms. It is practiced Significantly worldwide even though its t...
PATTERNS AND PREVALENCE OF CONGENITAL ANOMALIES AMONG NEONATES AND ASSOCIATED RISK FACTORS IN MISURATA CENTRAL HOSPITAL –LIBYA / 2015
Background: Congenital anomalies are defined as structural or functional abnormalities that present at birth. They are a major cause of neonatal stillbirths and neonatal morbidity and mortality. Objective: to determine t...
ANTIBACTERIAL ACTIVITY OF SOME MEDICINAL PLANTS AGAINST CAR-BAPENEM-RESISTANT ACINETOBACTER BAUMANNII ISOLATED FROM PATIENTS
Carbapenem-resistant Acinetobacter baumannii has currently emerged as a major threat leaving behind no antibac-terial treatment options. Therefore, current research has been focused on finding out effective natural plant...