EVALUATION OF CELL DEATH MECHANISMS INDUCED BY HYPERICIN AND ITS DERIVATIVE IN COLORECTAL CARCINOMA MODELS
Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2019, Vol 6, Issue 10
Abstract
Colorectal cancer (CRC) is the third most malignancy and the fourth human killer. Photodynamic therapy (PDT) has emerged as a promising alternative to conventional cancer therapies. This study aimed to investigate the ability of Hypericin (HY) and its derivative (HAHY) as synthetic photosensitizers (PSs) to induce tumoricidal effect in HCT-116 carcinoma models. Sixty balb/c male mice were transplanted with 5×105 HCT-116 cells to induce carcinoma bearing mice model. The experimental animals were then divided into five groups; Group I (untreated), Group II (HY-PDT), Group III (HAHY-PDT), Group IV (HY-PDT+EGCG) and Group V (FOLFOX). Blood, liver and tumor mass samples were taken. Also, biochemical studies and MTT assay were performed. HY and HAHY-PDT had a significant cytotoxic effect on human HCT-116 cells. Also, a significant upregulation in gene expression of P53 and CYT-c in concentration dose dependent manner. Furthermore, a significant increase in IL-6 gene expression in group (2) higher than that in group (3).Whereas it decreased in group (5), and showed insignificant difference in group (4) .Also, results showed decrease in VEGF gene expression in both group (3) and (4), while it showed significant increase in group (2) and slight increase in group (5). In conclusion, these PSs possess a strong cytotoxic and apoptotic effect on human HCT-116 cells. Using of EGCG along with HY-PDT improves the outcome. Also, HAHY-PDT can be used as an alternative to HY-PDT, where it showed tumor shutdown without metastasis besides its antitumor immunity.
Authors and Affiliations
Hala M. Ali
Keywords
Related Articles
- EP ID EP674557
- DOI -
- Views 149
- Downloads 0
SEROPREVALENCE OF HBV, HCV AND HIV AMONG VOLUNTARY BLOOD DONORS AT A TERTIARY HOSPITAL IN BAUCHI, NORTHEASTERN NIGERIA
Background; Blood donation and transfusion are an integral part of effective healthcare delivery and transmissible infections remain a public health problem. Post transfusion infection has its consequential effect on tra...
THE TREATMENT OF REFRACTORY NEPHROTIC SYNDROME IN CHILDREN
Idiopathic nephrotic syndrome is the commonest chronic kidney disease in children. Most of them are response to steroid treatment. However, about 20% patients are frequently relapse, steroid-dependent, or steroid-resista...
SYNTHESIS OF A NOVEL UNSATURATED POLYOLEFIN INTERMEDIATES, ALDEHYDE AND FATTY ACID
Novel unsaturated compounds with the required skip-conjugated (Z,Z)-double bonds configuration are synthesized through a coupling of aldehydes with phosphonium salt through a series of Wittig reactions as a main step. Th...
EVALUATION OF NITRIC OXIDE RADICAL SCAVENGING ACTIVITY OF A HEALTH SUPPLEMENT, HERBALONE
Nitric oxide (NO) plays a key role in the production of reactive nitrogen species (RNS). The objectives of this study were to determine antioxidant activities of ethanol extracts of raw materials and product samples were...
COMPARISON OF THE GENDER SPECIFIC HLA HAPLOTYPES DISTRIBUTION IN UZBEK PATIENTS WITH CHRONIC GLOMERULONEPHRITIS AND ESRD
Chronic glomerulonephritis (CG), chronic renal failure (CRF) are the majority of instances to end stage renal disease (ESRD) which is the cause of renal replacement therapy. Our interest was to evaluate the possible asso...