Abstract

Twenty five induced anaemic wistar rats of both sexes weighing 160-200g were used in this study to evaluate the effectiveness of consumption of mixed diet of garlic, garden egg and groundnut on the cytoarchitecture of the spleen. The animals were divided into five groups labelled 1, 2, 3, 4 and 5. Group 1 served as the non-anaemic control and was fed with normal rat chow. Groups 2, 3, 4 and 5 the experimental, were induced with anaemia by oral administration of phenylhydrazine (PHZ) given at 50mg/kgBW. Group 2 served as anaemic control, fed with normal rat chow; Group 3 were fed with 20g (75%ww) of garlic, garden egg and groundnut in the ratio of 1:1:1 with normal rat chow; Group 4 fed with 10g of garden egg (50%ww) in the ratio 1:1 with normal rat chow and Group 5 fed with 10g of groundnut (50%ww) in the ratio 1:1 with normal rat chow. Animals were sacrificed at the end of fourteen days of dietary feeding using chloroform inhalation method. The spleen were dissected out and fixed in 10% formal saline. Cytoarchitecture of spleen were observed in groups 1, 3 4 and 5 rats to be normal.. Sections from group 2 anaemic control rats, showed distortion of splenic white pulp with degenerated splenic nodules and diffused red pulp. Thus, the results from this study showed that the mixed diet of garlic, garden egg and groundnut have a protective effect on the cytoarchitecture of the spleen of induced anaemic wistar rats.

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