Evaluation of Culture Methods for Identification of Dermatophytes
Journal Title: Journal of Medical Science And clinical Research - Year 2014, Vol 2, Issue 10
Abstract
Dermatophytosisare of worldwide distribution. Epidemiological studies concerning dermatophytes infection have been performed and the differences in the etiological agents have been noted. The present study was undertaken to isolate & detect the etiological agents of dermatophytosis up to species level by microscopy & culture, & to evaluate the culture methods. A total of 72 clinically diagnosed untreated cases of dermatophytosis attending the Dermatology outpatient of a tertiary care hospital, Mysore, constituted the material for the study. Specimens such as hair, nail and skin scrapings were collected from suspected cases. Direct microscopy was done using potassium hydroxide preparation and culture was done on Saboraud’s dextrose agar and Dermatophyte test medium was done. Isolates were identified up to species level following conventional methods.72 samples from suspected cases of dermatophytosis were processed. Dermatophytes were isolated from 38 (52.77%) samples. Trichophyton rubrum was the commonest isolate 26 (36.11%). Male to female ratio was 2.3:1.21-40 years was the most common age group involved. Tinea corporis (22.22%) was the commonest clinical type of dermatophytosis. Conclusion: The present study provided data regarding the etiological agents of dermatophytosis in this part of Karnataka. DTM is very useful as a screening medium and the isolation of dermatophytes is rapid when compared to SDA. Early diagnosis is essential to prevent transmission of infection
Authors and Affiliations
Dr. Rajeshwari K Gangulappa
Keywords
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