EVALUATION OF CYTOTOXIC AND ANTHELMINTIC ACTIVITIES OF BARK EXTRACT OF APHANAMIXIS POLYSTACHYA (WALL.)
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 4
Abstract
Aphanamixis polystachya (Wall.) belongs to the family Meliaceae and it is extensively used in folkloric medicine of Bangladesh, for the treatment of various disorders. This evaluating study of methanolic extract of Aphanamixis polystachya bark was conducted to investigate the cytotoxicity, using brine shrimp lethality as test method as well as anthelmintic activity with the determination of time of paralysis and death using earthworm (Pheritima posthuma) at four different concentrations. The study confirmed that the methanolic extract of the bark of Aphanamixis polystachya possess mild cytotoxic activity (LC50=26.01±0.325 μg/ml) in comparison to the standard drug vincristine sulphate (LC50=0.839±0.013 μg/ml). On the other hand methanolic extract showed better anthelmintic activity as it required less time for paralysis and death comparing with the standard drug albendazole (concentration 10 mg/ml). At concentrations 10, 20, 40 and 60 mg/ml methanolic extract showed paralysis at mean time of 35.66± 0.72, 32.66±0.47, 27.66±0.72 and 25.66±0.27 and death at 52.33±0.72, 48.33±0.47, 40.00± 0.98 and 38.33±0.27 minutes, respectively. Whereas the standard drug albendazole showed paralysis and death at 56.20±0.20 and 77.40±0.24 minutes respectively. Thus, further studies are suggested to isolate the key compounds responsible for the cytotoxic and anthelmintic activities and also to conduct these tests using in vivo procedures.
Authors and Affiliations
Raihan Tanveer, Karmakar Palash, Abhijit Das, Banik Rana, Sattar Mafruhi
Keywords
Related Articles
- EP ID EP87450
- DOI 10.7897/2230-8407.04424
- Views 125
- Downloads 0
LABORATORY MODELS FOR SCREENING ANALGESICS
Pain is a complex unpleasant phenomenon composed of sensory experiences that include time, space, intensity, emotion, cognition and motivation. Analgesics are the agents, which selectively relieve pain by acting in the C...
EVALUATION OF ANTI DIABETIC ACTIVITY OF MARSILEA MINUTA LINN AGAINST ALLOXAN INDUCED DIABETES IN ALBINO RATS
The present study was design with an aim to evaluate the Ethanolic leaf extract of Marsilea minuta Linn (EEMM). The study was performed on ethanolic extract of MM leaf in oral glucose tolerance test (OGTT) and alloxan-in...
FORMULATIONS OF ISOSORBIDE MONONITRATE (ISMN) TABLET AND COMPARATIVE STUDY WITH AVAILABLE BRANDS
In the present study new formulations of Isosorbide mononitrate were manufactured by dry granulation method using Talc instead of magnesium stearate and compared with different brands of isosorbide mononitrate tablets av...
MICROBIOLOGICAL ASSAY FOR VITAMIN B
Micronutrients added to foods are analyzed using various procedures depending on their nature and properties. The microbiological assays are better than chemical method because any suitable change in vitamin molecule whi...
ISOLATION, PURIFICATION AND CHARACTERIZATION OF HEMIDESMUS INDICUS R.BR. ROOT EXTRACT
The roots of Hemidesmus indicus R.Br. was collected from Maruthamalai hills, Coimbatore and were extracted with 90% ethanol and fractionated by column chromatography using different solvents. A phytochemical Terpenoid wa...