Evaluation of DNA Damage in Type 1 Diabetes Patients
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 5
Abstract
ABSTRACT Introduction: DNA damage due to oxidative stress leads to progression of diabetic complications. The association between DNA damage and diabetes mellitus has prompted to study the extent of DNA damage and factors affecting this health problem in Type 1 Diabetes (T1D). Aim: To assess serum 8-hydroxy 2’deoxyguanosine (8-OHdG) levels as a DNA damage biomarker in T1D patients. Materials and Methods: The study was carried out between September 2017 and March 2018 at Duhok Diabetes Center, Duhok, Kurdistan Region (Iraq). Serum 8-OHdG levels of 132 T1D patients and 123 age and sex matched healthy control subjects were estimated using ELISA technique. The Insulin Autoantibodies (IAA), Islets Cell Antibodies (ICA) and Glutamic Acid Decarboxylase Antibodies (GADA) were assessed in T1D patients. Results: The results revealed that serum 8-OHdG levels were significantly raised in patients (6.02±2.05 ng/mL) as compared to healthy controls (2.03±1.63 ng/mL) (p<0.01). Mean 8-OHdG was also found to be significantly higher in patients with autoantibodies (6.20±2.12 ng/mL) as compared to patients without autoantibodies (5.7±1.93 ng/mL) (p=0.04). Moreover, the prevalence of DNA damage was found to be significantly higher in patients with autoantibodies (79.3%) as compared to patients without autoantibodies (40.0%) (p=0.02). Conclusion: The present study has indicated that DNA damage accompanied by autoantibodies is highly exhibited in patients with T1D. Antioxidant supplementation may be an effective public heath intervention to reduce DNA damage and oxidative stress.
Authors and Affiliations
Hivi Mohammed Mahmoud, Dhia J Altimimi
Keywords
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- EP ID EP583181
- DOI 10.7860/JCDR/2019/39851.12823
- Views 71
- Downloads 0
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