Evaluation of factions antigens disintegration fungal cells Candida albicans and Candida tropicalis if advised candidiasis
Journal Title: Фармацевтичний журнал - Year 2015, Vol 2, Issue 2
Abstract
Candidiasis – one of the most common fungal diseases humanity has ever faced. Etiological factors of the disease are most often Candida albicans and Candida tropicalis. To fight Candida infection has been actively conducting research on vaccine development, as in the CIS countries and in the countries of Europe and America. In the context of this research have shown trends of development of combined subunit vaccines based on the fungi Candida. Authors at the National University of Pharmacy at the Department of Biotechnology and Microbiology, Virology and Immunology developed a method of disintegration of cells of fungi C. albicans and C. tropicalis using ultrasound. The objective of our work was the experimental evaluation of fungal cell antigen fractions C. albicans and C. tropicalis in the prevention and treatment of candidiasis. Fungal cells from C. albicans and C. tropicalis were isolated proteins and polysaccharides using an ultrasonic disintegrator when extending wave of 22 kHz and 15 min exposure. Filtered through a membrane «Vladipor» IPA-MA № 3, which provides shutoff of the biological material with a size of 10 kDa.Obektom study were fractions dezintegrata fungal cell C. albicans and C. tropicalis molecular size and greater than 10 kDa. Mice were intramuscularly twice at an interval of 14 days were injected into the upper portion of the right hind paw of 0.2 ml of separately investigated fractions at a protein concentration for C. albicans 3 mg/ml for C. tropicalis 5 mg/ml. After 1 month, for one group, and after 3 months for a second group of test animals after the second injection, the animals carried abdominal infection. After 14 days the animals were carried out inspection and measured results The studies found that the fraction of cells dezintegrata fungi C. albicans and C. tropicalis with a molecular size of 10 kDa at double intramuscular injection of 0.2 ml provides 100% efficacy in the prevention of Candida infections.
Authors and Affiliations
M. V. Rybalkin, N. I. Filimonova, O. P. Strilets, L. S. Strelnikov
Keywords
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