Evaluation of Genetic Diversity in Citrus Genotypes by IRAP Molecular Marker
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 2
Abstract
It is important to identify the genetic diversity and phylogenic relationships for achieving desirable citrus cultivars. In present investigation IRAP markers were used to determine genetic diversity among 29 citrus genotypes. From 5 used primers, 49 polymorphic bands were amplified. Primers IRAP-1 and IRAP-2 (with 15 and 7 amplified bands), produced maximum and minimum polymorphic bands, respectively. Similarity among samples was calculated using NTsys software and Jaccard coefficient. Rang of similarity was 0.34-0.90 based on polymorphic bands with average of 0.65. Cluster analysis has been done based on Jaccard's similarity matrix and the UPGMA method. Cluster analysis has divided citrus genotypes into five separate groups. According to the similarity matrix results, the lowest similarity (0.34) was belonged to Dansi mandarin and Pommelo and the highest similarity (0.90) was belonged to unknown natural types G74 and Siavaraz orange 3 (G6). In current research Pommelo and mandarin confirmed as true species of citrus in distinct cluster.
Authors and Affiliations
Hajar Abedinpour, Gholam Ali Ranjbar, NadAli Babaein Jelodar and Behrouz Golein
Keywords
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