Evaluation of genetic diversity in different genotypes of (Medicago Sativa L.) using ISSR markers
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 22
Abstract
Alfalfa (Medicago sativa L.) is a widely grown legume and one of the most important forage species throughout the world. It yields forage of high nutritional quality and has positive influences on the environment. Genetic diversity based on different markers, plays a key role in breeding programs and one of the most important criteria for selection of Parents. In this study the genetic diversity among 19 genotypes of Medicago sativa was investigated using 8 ISSR primers. These primers could identify 37 loci. Primer IS5 showed the highest number of bonds(7) and primers IS1, IS9 and IS16 showed the lowest number of bonds(3). The Average percentage of polymorphism was 86% for primers used, that the lowest percentage of polymorphism was for IS14 and IS15 (50%) and IS5 (57.14%). Also it was 100% for other primers. The average of PIC for studied primers was 0.32 that highest rate of PIC belong to IS9 and IS13 and the primers IS5 and IS16 had the lowest. Also, cluster analysis and principal coordinate analysis using Dice similarity coefficients were calculated and dendrogram was drawn using the UPGMA for 19 genotypes. The coordinate analysis was performed using of the similarity matrix and confirmed the results of cluster analysis.
Authors and Affiliations
Fatemeh Bolourchian, Mohsen Farshadfar, Hooshmand Safari, Hooman Shirvani
Keywords
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