EVALUATION OF IAA AND PHAs PRODUCTION BY CHROMIUM RESISTANT BACTERIAL ISOLATES
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2018, Vol 6, Issue 3
Abstract
Extensive chromite mining activities have generated huge amount of toxic hexavalent chromium Cr (VI), which persists in the soil for many years. Long term accumulation of Cr(VI) in the soil decreases crop productivity in adjoining farming land. On account of that, 14 chromium resistant bacteria (CRB) were subjected to Indole Acetic Acid (IAA) and Polyhydroxyalkanoates (PHAs) Production. Bacterial strains such as Bacillus sp. CTSI-07, Enterobacter sp. CTWI-06 and Acinetobacter sp. CTWI-07 were producing 24, 114 and 106 µg/ml of IAA respectively. In addition, these bacterial isolates produced 0.75, 0.30 and 0.42 g/l of PHAs under submerged fermentation process. Moreover, higher amount of PHAs production (21.42%) was exhibited by Bacillus sp. CTSI-07. The extracted biopolymer is polyhydroxybutyrate (PHB) (most common homopolymer of PHAs) as revealed from structural characterization. As these bacterial strains have the capability to produce IAA and PHAs, which may be utilized for long term bioremediation of Cr(VI) in chromium contaminated soil as well as to maintain soil fertility.
Authors and Affiliations
S. Pattnaik , D. Dash; D. P. Samantaray
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