Evaluation of in vitro Antifungal Activity of Silver and Selenium Nanoparticles against Alternaria solani Caused Early Blight Disease on Potato
Journal Title: Biotechnology Journal International - Year 2016, Vol 12, Issue 3
Abstract
Aim: This study investigated the effect of silver and selenium nanoparticles on Alternaria solani, the pathogenic fungus causing early blight disease of potato. Place and Duration of Study: Drug Radiation Research Department, National Centre for Radiation Research & Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt, 2013. Methodology: The fungus was isolated from infected potato leaves that showed brown circular spots as early blight disease symptoms. Silver nanoparticles (AgNPs) were prepared biologically using gamma irradiated Trichoderma viride cell free supernatant. Selenium nanoparticles (SeNPs) were prepared by glutathione method. The synthesized AgNPs were characterized by UV-Vis spectroscopy, Dynamic Light Scattering (DLS) and Transmission Electron Microscope (TEM). Kocide® fungicide was used as reference. Results: The fungus isolated of leaf spot was identified both microscopically and genetically as Alternaria solani causing early blight disease of potato. AgNPs were spherical in shape with average size of 12.7 nm. Selenium nanoparticles were prepared by glutathione as reducing agents. Under laboratory conditions, 25 µg/ml concentration of silver nanoparticles completely inhibited A. solani as compared to Kocide®, fungicide that gave maximum inhibition at 600 µg/ml. The selenium nanoparticles completely inhibited the fungal growth at 800 µg/ml. Conclusion: AgNPs completely inhibited the growth of A. solani at low concentrations. Silver nanoparticles might be suitable alternative to chemical fungicides. While, SeNPs can be used as antioxidant for enhancing plant immunity.
Authors and Affiliations
Ahmed I. El-Batal, Abdel-Wahab A. Ismail, Nagwa M. Sidkey, Rawhia A. Arafa, Rasha M. Fathy
Keywords
Related Articles
- EP ID EP237822
- DOI 10.9734/BBJ/2016/24155
- Views 78
- Downloads 0
Improved Sample Preparation for PCR-Based Assays in the Detection of Xanthomonads Causing Bacterial Leaf Spot of Tomato
Aims: To develop a sampling procedure for PCR-based screening of bacterial leaf spot (BLS)-causing xanthomonads without DNA extraction from infected tomato plants. Place and Duration of Study: University of Copenhagen, D...
Kerosene: A Study of Serum Vitamin Levels of Female Wistar Rats Chronically Exposed
Background: Kerosene is a commonly available product used for cooking and lighting purposes in many parts of Asia and Africa where it is sold in beverage bottles and jerry cans in both commercial and residential places b...
Catalyzed Mediator-Based Decolorization of Five Synthetic Dyes by Pleurotus ostreatus ARC280 Laccase
Aim: The aim of the present study was to evaluate qualitatively the decolorization of five dyes by Pleurotus ostreatus (P. ostreatus) ARC280 using solid medium. The laccase produced by the fungus was used in terms of its...
Optimization of Cellulase (E.C. 3.2.1: 4) Production Using Penicillium citrinum MTCC 9620 in Solid State Fermentation
Aim: The objective of the present work was to optimize the environmental parameters for Cellulase (1,4 β-endoglucanase, E.C.3.2.1:4) production using Penicillium citrinum MTCC 9620 in Solid State Fermentation. Study Desi...
Molecular Identification of Cassava Mosaic Begomoviruses Associated with Cassava Mosaic Disease in the DR-Congo Using Primer Pairs
Molecular analysis of cassava leaf samples from different regions of the Democratic Republic of Congo (DR-Congo) was conducted to identify cassava mosaic begomoviruses (CMBs) associated with cassava infections in farmer...