Molecular Identification of Cassava Mosaic Begomoviruses Associated with Cassava Mosaic Disease in the DR-Congo Using Primer Pairs
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 5
Abstract
Molecular analysis of cassava leaf samples from different regions of the Democratic Republic of Congo (DR-Congo) was conducted to identify cassava mosaic begomoviruses (CMBs) associated with cassava infections in farmers' fields in Gandajika (Eastern Kasai), Bas-Congo and Kinshasa. Four specific primer pairs M1F/M1R, M2F/M2R, Begomo 146/Begomo 672, and EAC4F/EAC4R were used to detect East African cassava mosaic virus (EACMV) AC2, African Cassava Mosaic Virus (ACMV) AC2, ACMV DNA-A, and EACMV-UG DNA-A AC4, respectively. Based on PCR analysis, only African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV-UG) were identified in the cassava growing regions. Overall, 67% of cassava samples were infected with ACMV, 10% with EACMV-UG, and 10% with both, ACMV and EACMV-UG. No virus was detected in 13% of symptomatic samples. Thus, although EACMV-UGG is present in targeted areas, ACMV remained the most common CMB in the DR-Congo.
Authors and Affiliations
Kabwe K. C. Nkongolo, Marcel Muengula-Manyi, Claude Bragard, Adrien Kalonji-Mbuyi, Djim Tshilenge-Kanana
Keywords
Related Articles
- EP ID EP237326
- DOI 10.9734/BBJ/2014/8112
- Views 105
- Downloads 0
Antibacterial Activity of Ocimum gratissimum (Nchu-Anwu) and Vernonia amygdalina (Bitter-Leaf) Antibacterial Activity of Ocimum gratissimum (Nchu-Anwu) and Vernonia amygdalina (Bitter-Leaf)
This study was carried out to evaluate the antibacterial activity of Ocimum gratissimum and Vernonia amygdalina on selected bacterial species, as well as comparing the antibacterial activities of their fresh and dried le...
Plasmid and Restriction Endonuclease Analyses of Bacteria Associated with Pneumonia in HIV/AIDS Patients in Ekiti State, Nigeria
Bacterial infections are an important cause of morbidity and mortality in individuals with the human immunodeficiency virus. The emergence of multiple-drug resistant bacteria has been documented by many researches. This...
Characterization and Antimicrobial Susceptibility Patterns of Isolates from Ward Fomites
Aim: The study was conducted to determine antimicrobial susceptibility patterns among isolates from ward fomites at Kiwoko Hospital and to detect resistances in the form of Macrolide Lincosamide StreptograminB (MLSB), Me...
In vitro Evaluation of the Effect of Inorganic Fertilizer on Rhizosphere Soil Microbial Populations during Early Growth of Zea mays and Phaseolus vulgaris
Aims: The study assessed the effects of different regimen of inorganic fertilizers on the microbial community structure in the rhizosphere soil of Zea mays and Phaseolus vulgaris during early growth. Study Design: Seeds...
Prokaryotic Diversity of the Alkaline Lake Acıgöl, Turkey by Using Culture-dependent and Culture-Independent Methods
Aims: The prokaryotic diversity of Acıgöl lake, Turkey, was studied in samples taken from soil, water and mud. Culture -dependent and -independent methods were used to analyse the results. Methodology: Bacterial cultures...