EVALUATION OF IN VITRO ANTIOXIDANT AND ANTI-PROLIFERATIVE EFFECT OF DEFATTED EXTRACT OF COCOS NUCIFERA KERNEL ON HUMAN BREAST CANCER CELL LINE
Journal Title: International Journal of Pharmacognosy - Year 2018, Vol 5, Issue 3
Abstract
The present study was designed to evaluate the in vitro antioxidant effects and potential benefits of using coconut kernel extract in preventing the proliferation of human breast cancer cell line, MCF-7. Defatted extract from coconut kernel (CKf) was evaluated for its radical scavenging, prevention of human LDL oxidation, cytotoxic, apoptotic and nuclear effects on MCF-7 cells. Intracellular production of reactive oxygen species (ROS) and superoxide radical were studied. It was showed that CKf significantly scavenged the free radicals and prevented LDL oxidation. MCF-7 cell viability was found to be decreased in a dose dependent manner following treatment with CKf. AO-EB and DAPI staining confirmed that treatment with CKf, induced nuclear damage and apoptosis as distinctly visualised in cells, Our observations suggest that nuclear/cellular damage and poor mitochondrial membrane potential (MMP) appears to be an indirect consequence of the excessive generation of reactive oxygen species (ROS) and superoxide radicals.
Authors and Affiliations
Kottayath Govindan Nevin et al.
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