EVALUATION OF IN VITRO ANTIOXIDANT AND ANTIDIABETIC POTENTIALS OF DIFFERENT FRACTIONS OF MAYTENUS HEYNEANA ROOT EXTRACT
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: In an attempt to explore herbal drug which may become useful in the prevention of diabetes and antioxidant potential by the ethanol extracts of Maytenus heyneana (MH) root belonging to the family Celastraceae and their different fractions were studied.Methods: Different fractionation was done using chloroform, ethyl acetate, and methanol on ethanolic extract of MH and preliminary phytochemical analysis was done by standard methods to identify the presence of important compounds. In vitro antioxidants activities were carried by 2,2-diphenyl- 2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation radical scavenging assays. For antidiabetic potential, α-amylase and α-glucosidase enzyme inhibitory studies were carried on different fractions.Results: Phytochemical studies show the presence of alkaloids, flavonoids, phenols, cardiac glycosides, and terpenoids in all fractionations; however, tannins and quinones were present in ethyl acetate fraction and saponins in methanolic fraction. For antioxidant activity, ethyl acetate fraction shows concentration of the sample causing 50% inhibition (IC50) values in 22.31 μg/ml and methanolic fraction shows in 12.82 μg/ml concentrations for DPPH and ABTS radical scavenging assay, respectively. In case of antidiabetic activity, methanolic fraction offered significant result in inhibitory action of α-glucosidase and also for α-amylase assay IC50 (5.28 and 3.14 μg/ml) than other fractions.Conclusion: From the results of our studies, it can be concluded that MH shows antidiabetic and antioxidant values and methanolic fraction of MH could be possessed potential constituents in the prevention of diabetes and antioxidant than other fractions. However, further studies are required to validate.
Authors and Affiliations
Sumithira G, Senthil Kumar Gp
Keywords
Related Articles
- EP ID EP607172
- DOI 10.22159/ajpcr.2019.v12i1.30088
- Views 101
- Downloads 0
ANTIPROLIFERATIVE AND APOPTOTIC EFFECTS OF FICUS PSEUDOPALMA BLANCO (MORACEAE) AGAINST HEPATOCARCINOMA (HepG2)
Objectives.Hepatocellular carcinoma is the third most frequent cause of cancer-related death in the Philippines. The prevalence of this disease continuously pose a great challenge to the medical and health sectors of t...
EVALUATION OF ANXIOLYTIC EFFECT OF MELILOTUS OFFICINALIS EXTRACTS IN MICE
Objective: Anxiety is one of the most common and serious mental illness affecting humankind and its extensiveness is on the rise at an alarming rate. Anxiolytic substances are highly acclaimed in the ranking of the most...
PREPARATION OF MUCOADHESIVE GASTRORETENTIVE DRUG DELIVERY SYSTEM OF ALGINATE BEADS CONTAINING TURMERIC EXTRACT AND ANTI-GASTRIC ULCER ACTIVITY
Objectives: The objectives of this study were to prepare the mucoadhesive mucoadhesive gastroretentive drug delivery system of alginate beads containing turmeric extract and to evaluate its anti-gastric effect.Methods: T...
PHYTOCHEMICAL ANALYSIS AND EVALUATION OF ANTIMICROBIAL ACTIVITY IN THE WHOLE PLANT EXTRACTS OF GLORIOSA SUPERBA
Objectives: The aim of this research work was to carry out the phytochemical screening and to evaluate the antibacterial and antifungal activity of the whole plant extract (shoot, flower, and tuber) of the flame lily (Gl...
EFFECT OF NERVE CONDUCTION STUDIES IN HYPOTHYROIDISM - AN OBSERVATIONAL STUDY
Objective: This observational study was conducted during the year 2016–2017 to assess the electrodiagnostic evidence of peripheral nerve dysfunction among newly diagnosed hypothyroid patients attending a tertiary care...