Evaluation of Molecular Technique over Conventional Culture Method for Early Diagnosis of Helicobacter pylori Infection
Journal Title: International Journal of Medical Research Professionals - Year 2018, Vol 4, Issue 3
Abstract
Background: Helicobacter pylori infection is an important triggering agent for development of gastric malignancies, peptic ulcer disease and other dyspeptic symptoms. Therefore a crucial part in the management of infection is a fast and accurate diagnostic technique which would give reliable results in detecting infection in symptomatic patients. Aim: This study aimed to compare and evaluate the reliability of molecular technique over conventional culture method for early diagnosis of H. pylori infection in dyspeptic patients. Materials and Methods: A cross sectional hospital-based study was conducted between June 2016 and May 2017 and included 100 gastric biopsy specimens from clinically suspected dyspeptic patients undergoing routine upper gastro-intestinal tract endoscopy. These specimens were analyzed using the conventional culture method as well as molecular technique (PCR assay) for species identification and virulent factor determination directly from biopsy specimens. Results: The molecular method could detect H. pylori in 44% cases with detection virulent cagA and vacA genes, whereas the culture method could detect 20% cases. Moreover the turnaround time taken by molecular method was 24-36 Hours whereas that of the culture method was 5-6 days. Conclusion: The molecular method can be considered as a more sensitive as well as rapid method for detection of H. pylori from biopsy specimens which can be used routinely for diagnosis of H. pylori infection.
Authors and Affiliations
Anisha Sarma, Lahari Saikia, Mituban Gogoi
Keywords
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- EP ID EP531839
- DOI 10.21276/ijmrp.2018.4.3.017
- Views 62
- Downloads 0
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