Evaluation of nucleic acid testing [NAT] of blood donors
Journal Title: Indian Journal of Pathology and Oncology - Year 2018, Vol 5, Issue 4
Abstract
Context Human immunodeficiency virus HIV hepatitis C virus HCV and hepatitis B virus HBV are easily transmitted through infected blood to prevent this serological screening of blood units is a routine norm Many cases could be missed if the testing is done in window period To overcome this and to provide an additional protection many tests with better sensitivity and specificity like Nucleic acid testing NAT have been triedAims To observe NAT reactivity trend for transfusion transmittable diseases and to correlate ELISA and NAT methods in detecting HIV HBV and HCVMaterials and Methods A six years retrospective observational study conducted in Blood bank Department of Pathology ELISA and IDNAT results of the donor samples for HIV HBV and HCV were collected in a specially designed proforma and analyzedStatistical Analysis used Data entered in Microsoft excel sheets means and percentages were calculatedResults In 6years 52417 units of blood collected 446008508 were voluntary donors and 78171492 replacement donors There were 521099 cases of HBV 98 019 HIV and 48 009 HCV and two cases of HIVHBV coinfection About 557 were positive by both ELISA and NAT method 106 Fifty eight cases were positive only by IDNAT method and negative on serological testing accounting to the NAT yield of 1 in 893 cases There were 2 cases of HIV2 which were missed by NAT and picked up in ELISAConclusions Screening of blood and blood products using dual testing with high sensitivity serological assays and NAT helps to detect potentially infectious diseases in all phases of infectionKeywords IDNAT ELISA Transfusion transmittable diseases
Authors and Affiliations
Chaithanya K, Shivakumar S
Keywords
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- EP ID EP475906
- DOI 10.18231/2394-6792.2018.0104
- Views 57
- Downloads 0
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