EVALUATION OF RAPID UREASE TEST IN DIAGNOSIS OF Helicobacter pylori AMONG GASTRODUDENITIS AND PEPTIC ULCER Patients IN COMPARISON WITH PCR IN KHARTOUM STATE
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2015, Vol 4, Issue 3
Abstract
Background: Recently Helicobacter pylori (H. pylori) has been involved in many serious diseases such as peptic ulcer and ended with gastric cancer, which can be prevented using specific diagnostic test and treatment. Objective: The aim of this study was to evaluate rapid urease test (RUT) using Polymerase Chain Reaction (PCR) as gold standard method, for the detection of the H. pylori in gastric and duodenal biopsy specimens of gastrodudenitis and peptic ulcer, in Khartoum state, Sudan. Materials and Methods: Fifty patients with gastrodudenitis and peptic ulcer attending Alribat Teaching hospital during period from March to April were enrolled in this study. Two gastric/duodenal biopsies specimens were collected and subjected for RUT and PCR for detection of 16-S gene. Sensitivity, specificity of RUT was calculated using PCR as gold standard. Results out of 50 specimens tested 26 were positive (52%) and 24 were negative (48%) by using PCR. Out of 26 positive by PCR 22 were positive by RUT and out 24 negative by PCR 21 were negative by RUT .The sensitivity, specificity of RUT were 84.6% and 87.5%, respectively. Conclusion in Comparison with PCR , RUT show high specificity and sensitivity. So its use will serve as good alternative to PCR particularly in resources limited area.
Authors and Affiliations
Waad Abdelmonem Elsheikh Ahmed| Department of medical microbiology, faculty of medical laboratory sciences, Alneelain University., Mustafa E Yassin| Department of medical laboratory, faculty of Medical Technology Surman, Zawia University, Libya., Mohanad Eltahir Ismail Mosa| Department of medical microbiology, faculty of medical laboratory sciences, Alneelain University., Wafa Ibrahim elhag| Department of medical microbiology, faculty of medical laboratory sciences, Alneelain University., Abbas Bakhit Mohamed Rahama| Department of medical microbiology, faculty of medical laboratory sciences, Alneelain University., Aymen Awad Abdelhaleem| Department of Molecular Biology & Immunology, Medical Research Center MRC University of Jazan, K.S.A., Fatima Hussein salim| Department of endoscopy, Alribat Teaching hospital, Khartoum, Sudan.
Keywords
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