Evaluation of Sperm Viability and Acrosomal Integrity by Flow Cytometry Analysis in Jersey Crossbred Bulls
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 10
Abstract
Flow cytometry based evaluation of sperm parameters is advantageous over the traditional microscope based evaluation. Many sperm parameters can be measured within short time period and with high accuracy. For this study, semen from six Jersey crossbred bull were collected. Semen parameters like sperm for viability and acrosomal integrity were analyzed by flow cytometry. Sperm for viability measured by the propidium iodide staining indicated that Bull No: 3 have the highest number of viable spermatozoa with 82.89 per cent followed by 82.18, 80.26, 79.43, 75.20 and 70.73 for Bull No: 1, 5, 2, 6 and 4 respectively. Acrosomal integrity of the sperm sample were determined by fluorescein isothiocynate (FITC) labelled with Peanut agglutinin (PNA) staining. The percentage of viable sperm, non-viable sperm, viable intact sperm, viable damage sperm and viable damage and non-viable sperm was ranged from 75.12 to 82.28, 17.72 to 30.66, 58.55 to 78.48, 3.80 to 10.80 and 21.52 to 41.45 respectively. Chi’s square analysis of the semen samples indicated that there was a significant difference among bulls with respect to both the sperm viability and acrosomal integrity (P<0.01).
Authors and Affiliations
V. Rajaram, A. Palanisammi, S. N. Sivaselvam, K. Kumanan
Keywords
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- EP ID EP572997
- DOI 10.20546/ijcmas.2017.610.010
- Views 91
- Downloads 0
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