Evaluation of the effect of the generated conditions by temporary immersion Bioreactors on enzymes and key processes of carbon metabolism in in vitro plants of plantain cv. CEMSA ¾
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 3
Abstract
The technique of Temporary Immersion constitutes an effective tool for the plant propagation; it increases the multiplication coefficient and the quality of the same ones. Little it is known until the moment about the echophysiology of this new cultivation technique where the plants are subjected to an immersion between liquid and the physiologic changes that take place during this stage will be those responsible for the plants quality. To evaluate the effects of these conditions in the physiologic change of plantain plants, a descriptive experiment was directed during the elongation phase. Enzymes related with the carbon metabolism were measure of the plant heterotrophic behaviour during the studied phase. High levels of acid invertase (AI) activities and pyruvato kinase (PK) accompanied by first floor levels of sucrose phosphate synthase (SPS) and phosphoenolpyruvate carboxylase (PEPC), evidence these behaviours. The changes induced by the atmosphere and the indicative net photosynthesis and transpiration demonstrate the tendency from the plants to be a less autotrophy systems. The plants during the in vitro conditions don’t depend totally on the photosynthesis but rather they present photomixotrophic behaviour.
Authors and Affiliations
Carlos E. Aragón, Maritza Escalona, Iris Capote, Danilo Pina, Inaudis Cejas, Justo González-Olmedo
Keywords
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