Evaluation of the effectiveness of bleach on microbial population of lettuce
Journal Title: Scientific Journal of Microbiology - Year 2013, Vol 2, Issue 9
Abstract
The effectiveness of bleach on the microbial population of lettuce was evaluated. Microorganisms on lettuce before and after washing with bleach were searched and counted and a new dose for cleaning to eliminate a high concentration of bacteria on the lettuce has been proposed to minimize the health risk linked to its consumption. The lettuce samples were collected from farms municipalities V and VI of the District of Bamako. Ten lettuces were collected from different plots and mixed to form a composite sample. The concentration of microbial population was determined by finding and counting the total microflora in 100g of lettuce composite sample before and after washing with bleach different chlorine concentrations for different contact times. Lettuces grown in Bamako are heavily contaminated with microbial population average between 25 to 1363UFC / g of lettuce. The application of the recommended dose of chlorine (133.10-5mg / l) in Mali for the disinfection of fruits and vegetables for 15, 30 and 60 min resulted in a drawdown respectively 94%, 97% and 99% of the initial microbial population lettuce. The recommended dose and double dose of this leading to high rates of drawdown of 97% and 99% of the initial microbial population of lettuce. 1% of the concentration of microbial population remains on the treated leaves. Dose which totally eliminates the microflora is 2.6 mg / l of chlorine applied for fifteen minutes. The new dose we can offer is the application of 2.6 mg / l of active chlorine.Keywords: Lettuce, bleach, efficiency, disinfection, concentration of microbial population.
Authors and Affiliations
S. Traoré*| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., F. Samaké| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., A. H. Babana| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., D. Traoré| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., Y. Sanogo| Laboratory of Applied Molecular Biology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206., K. Maiga| Research Laboratory of Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Science, Technique and Technology Bamako, BPE. Bamako-Mali 3206.
Evaluation of the sanitary quality of peanut butters from Mali: Identification and quantification of Aflatoxins and pathogens
InMali, peanut remains a major oilseed crops and constitutes in various forms avery important source of fat and protein for the Malian people. Despite theimportance of peanut butters in the diet in Mali and the potential...
Inhibitory effect of Carum carvi essential oil on the growth of Candida albicans
Candida albicans is the opportunistic yeast which causes serious health problems in human and animals. Resistance of C. albicans against many commercial drugs has make it necessary to explore natural resources for findin...
Seroprevalence of measles IgM in children 5-12 years from selected primary schools in Giwa Local Government Area, Zaria, Kaduna state
Seroprevalence of Measles IgM was carried out among children age 5-12 years in Giwa, Kaduna State. The test was carried out using Enzyme Linked Immunosorbent Assay (ELISA) method. Of the 90 (100%) samples assayed for the...
Uropathogens from diabetic patients with asymptomatic bacteriuria and Urinary tract infections
Urinary tract infections occur more frequently in diabetic than in non-diabetic patients and have been proven to be the primary cause of renal failure in diabetics. This study was to investigate the prevalence of asympto...
Promotion and implementation of bioenergy for a better environment: A mini review
There is strong scientific evidence that the average temperature of the earth’s surface is rising and this may be attribute to increased concentration of carbon dioxide (CO2), and other greenhouse gases (GHGs) in the atm...