Evaluation of the Effects of a High Dose of Erythropoietin-beta on Early Endotoxemia Using a Rat Model
Journal Title: Advances in Clinical and Experimental Medicine - Year 2012, Vol 21, Issue 3
Abstract
[b]Abstract[/b] [b]Background.[/b] Endotoxins can cause serious organ damage and death by triggering the secretion of pro-inflammatory cytokines such as TNF-α, IL-6 and IL-1β in bacterial infections. [b]Objectives. [/b]The goal of this study was to evaluate the effects of a high dose (3000 U/kg) of erythropoietinbeta (EPO) on inflammatory cytokine levels, renal function and histological changes during the early period of Lipopolysaccharide (LPS)-induced endotoxemia using a rat model. [b]Material and Methods.[/b] Male Sprague Dawley (350–400 g) rats were randomized into 3 groups: Control group (n = 7); LPS group (received 20 mcg/kg LPS through intraperitoneal (i.p.) injection (n = 7); LPS+EPO group (received 3000 U/kg, ip 30 minutes before LPS administration (n = 7). Four hours after the administration of LPS, kidney tissue and serum samples were collected. Kidney function parameters, TNF-α, IL-6, IL-1β, C reactive protein (CRP) and complete blood counts (CBC) were measured. The severity of renal tubular injury and caspase- 9 immunoreactive cells was expressed as a percentage. [b]Results.[/b] Serum levels of urea, creatinine, TNF-α, IL-6 and IL-1β were significantly increased in the LPS group (p < 0.0001 – p = 0.04) and were lower in LPS+EPO group (p < 0.0001, p = 0.01, p = 0.02, p = 01 and p < 0.0001, respectively). Pretreatment with EPO significantly increased platelet counts (p = 0.00) and decreased white blood cell counts (p = 0.02). The renal tubular injury percentage was significantly higher in the LPS group than in the control and LPS+EPO groups (p = 0.002, p = 0.003, and p = 0.005, respectively) and caspase-9 expression was lower in the LPS+EPO and control groups than in the LPS group. [b]Conclusions.[/b] EPO might have renoprotective effects against the inflammatory process and cell apoptosis during endotoxemia.
Authors and Affiliations
Zehra Eren, Jale Çoban, Işın Ekinci, Cigdem Kaspar, Gülçin Kantarcı
Keywords
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