Evaluation of the In Vitro Cytotoxic Activity of Jania rubens Against Jurkat and Molt-4 Human Cancer Cell Lines
Journal Title: Tropical Journal of Natural Product Research - Year 2017, Vol 1, Issue 2
Abstract
Cancer is a malignant disease that is characterized by rapid and uncontrolled proliferation of abnormal cells which may accumulate together to form a tumour mass or diffuse throughout the body. It is second to heart disease among the leading noncommunicable diseases and is a major threat to mankind. The present study aims at the preliminary phytochemical screening and cytotoxic evaluation of methanol extract of Jania rubens against Jurkat and molt-4 human cancer cell lines. The algae Jania rubens Linn was collected manually from East Coast of Bay of Bengal. Different concentrations (0, 1.953, 3.908, 7.8125, 15.625, 31.25, 62.5, 125, 250, 500 and 100 µg/mL) of the methanol extract from Jania rubens were prepared and tested for its potential cytotoxic activity against Jurkat and molt-4 human lymphoblastic leukaemic cell lines. The cells were treated with different concentrations of the algal extract and the cytotoxicity of the extract was evaluated by methyl thiazolyl tetrazolium (MTT) assay. Also, the number of viable cells was determined by trypan blue test. IC50 values of the algal extract against the cell lines were found to be 62.5 and 60.25 µg/mL, respectively. The methanol crude extract of the algae Jania rubens exhibited moderate cytotoxic activity against Jurkat and Molt-4 cell lines and may be a good source of anticancer agents against leukaemia.
Authors and Affiliations
Rahamat Unissa
Keywords
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- EP ID EP349914
- DOI 10.26538/tjnpr/v1i5.5
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