Evaluation of the uncommon bacterial pathogens in urogenital infection of patients referred to Iran Hospital with phenotypic and molecular methods
Journal Title: Journal of Alternative Veterinary Medicine - Year 2020, Vol 3, Issue 7
Abstract
Background and aim: One of the most common infections, especially nosocomial infections in humans, is urinary tract infection (UTI). UTI-causing agents include bacteria, fungi and viruses. Bacteria, especially Escherichia coli with about 85% is the most common cause of this type of infection. Other bacteria include Klebsiella, Enterococcus, Pseudomonas, Staphylococcus, Proteus, Neisseria, Mycoplasma and Chlamydia. The aim of this study is evaluation the prevalence of uncommon bacteria in the incidence of UTI using phenotypic and molecular (PCR) methods detection. Materials and methods: In this study, during six months, 235 urine samples were collected from patients referred to Iran Hospital who had UTI symptoms, and simultaneously with microbial culture in the laboratory, DNA extraction was performed using extraction kits and PCR test with specific primers to detect these bacteria in each Samples were taken. Results: In the samples, 137 samples (58.29%) contained uncommon UTI bacteria. The prevalence of uncommon bacteria was as follows: Pseudomonas aeruginosa 74 samples (31.4%), Enterococcus faecalis 61 samples (25.9%), Klebsiella pneumoniae 32 samples (13.6%), Staphylococcus saprophyticus 2 Sample (10.2%), Proteus Myrabilis 13 specimens (5.5%), Neisseria gonorrhoea 7 specimens (2.9%), Chlamydia trachomatis and Mycoplasma 5 specimens each (2.1%). Only 19.7% of samples containing uncommon bacteria were detected by routine laboratory methods (phenotypic diagnosis). Conclusion: The prevalence of these bacteria varies according to the geographical location, level of health, health control and level of culture of each country. Delay in accurate identification of these bacteria increases bacterial resistance to antibiotics prescribed by physicians.
Authors and Affiliations
Amir Emami, Seyed Reza Khedmati
Keywords
Related Articles
- EP ID EP698430
- DOI -
- Views 108
- Downloads 0
An Investigation into the Effects of Aqueous Extract of Carum Copticum on Renal Tissue and Uric Acid in Male Wistar Rats
Of the nine chemical compounds identified in the Carum Copticum extract, thymol, γ-terpinen, and parasaymn are considered as the main compounds utilized as antiemetic, anti-asthma, anti-nausea, and spasmodic, locally use...
External Parasitic Worms and Protozoans of the Fish Caught in Shapour River and Seyyed Hossein Spring in Kazerun, Iran
In this study, 38 fish in four species of Shapour River and Seyyed Hossein Spring were caught in 2016, including: Albumus mossulensis, Cyprinion macrostomum, Capoeta barroisi persica and Garra rufa and they were studied...
Evaluation of PCR Assay Using Specific Primers in Diagnosis of Canine Ehrlichiosis and Babesiosis:A Study on Herd and Stray Dogs in Shiraz
Canine tick-borne diseases are regarded as emerging problems within Iran and throughout the world. The present article aims at investigating Ehrlichia canis and Babesia canis infections in herd and stray dogs in Iran. Th...
The effect of Trachyspermum optical aqueous extract on blood Homeostasis
T. capsicum from medicinal herbs is considered as a rich source of thymol with healing properties. Enzymatic coagulation factors stem from the liver. In the present study, 48 adult Wistar male rats were selected and divi...
An Evaluation of Antimicrobial Effects of Zosima Absinthifolia Extracts
For the purpose of this study, the antimicrobial effects of Zosima absinthifolia extract were tested on Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Dried seeds of Zosima absinthifolia plant (100 g)...