Evaluation of the uncommon bacterial pathogens in urogenital infection of patients referred to Iran Hospital with phenotypic and molecular methods
Journal Title: Journal of Alternative Veterinary Medicine - Year 2020, Vol 3, Issue 7
Abstract
Background and aim: One of the most common infections, especially nosocomial infections in humans, is urinary tract infection (UTI). UTI-causing agents include bacteria, fungi and viruses. Bacteria, especially Escherichia coli with about 85% is the most common cause of this type of infection. Other bacteria include Klebsiella, Enterococcus, Pseudomonas, Staphylococcus, Proteus, Neisseria, Mycoplasma and Chlamydia. The aim of this study is evaluation the prevalence of uncommon bacteria in the incidence of UTI using phenotypic and molecular (PCR) methods detection. Materials and methods: In this study, during six months, 235 urine samples were collected from patients referred to Iran Hospital who had UTI symptoms, and simultaneously with microbial culture in the laboratory, DNA extraction was performed using extraction kits and PCR test with specific primers to detect these bacteria in each Samples were taken. Results: In the samples, 137 samples (58.29%) contained uncommon UTI bacteria. The prevalence of uncommon bacteria was as follows: Pseudomonas aeruginosa 74 samples (31.4%), Enterococcus faecalis 61 samples (25.9%), Klebsiella pneumoniae 32 samples (13.6%), Staphylococcus saprophyticus 2 Sample (10.2%), Proteus Myrabilis 13 specimens (5.5%), Neisseria gonorrhoea 7 specimens (2.9%), Chlamydia trachomatis and Mycoplasma 5 specimens each (2.1%). Only 19.7% of samples containing uncommon bacteria were detected by routine laboratory methods (phenotypic diagnosis). Conclusion: The prevalence of these bacteria varies according to the geographical location, level of health, health control and level of culture of each country. Delay in accurate identification of these bacteria increases bacterial resistance to antibiotics prescribed by physicians.
Authors and Affiliations
Amir Emami, Seyed Reza Khedmati
Keywords
Related Articles
- EP ID EP698430
- DOI -
- Views 104
- Downloads 0
An Evaluation of Antimicrobial Effects of Zosima Absinthifolia Extracts
For the purpose of this study, the antimicrobial effects of Zosima absinthifolia extract were tested on Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Dried seeds of Zosima absinthifolia plant (100 g)...
External Parasitic Worms and Protozoans of the Fish Caught in Shapour River and Seyyed Hossein Spring in Kazerun, Iran
In this study, 38 fish in four species of Shapour River and Seyyed Hossein Spring were caught in 2016, including: Albumus mossulensis, Cyprinion macrostomum, Capoeta barroisi persica and Garra rufa and they were studied...
Radiographic study of navicular bone in hind feet of sound Dareh-shori horses
Navicular abnormalities has long been considered one of the most common causes of fore limb lameness. Radiographic evaluation is one of the most valuable, economic, and initial diagnostic tools used in the evaluation of...
Evaluation of the uncommon bacterial pathogens in urogenital infection of patients referred to Iran Hospital with phenotypic and molecular methods
Background and aim: One of the most common infections, especially nosocomial infections in humans, is urinary tract infection (UTI). UTI-causing agents include bacteria, fungi and viruses. Bacteria, especially Escherichi...
The Effect of Ginger on the Growth of Lactobacillus Acidophilus and Bifidobacterium Bifidum in Probiotic Dairy Products
Probiotics are regarded as live microorganisms that provide health benefits when consumed. In the present study, the potentiality of producing foods containing probiotic bacteria with milk, yoghurt and ginger bases was e...