Isolation and molecular Identification of Streptococcus mutans and evaluation of the antimicrobial effect of some herbal plant extracts on them
Journal Title: Journal of Alternative Veterinary Medicine - Year 2020, Vol 3, Issue 7
Abstract
Introduction and aim: Overuse of antibiotics culminated in increasing of frequency of occurrence of antibiotic resistant bacteria. Therefore, new drugs with minimum complication are needed. Nowadays, herbal drugs as new remedy have been considered to reduce frequency of occurrence of antibiotic resistant bacteria. The purpose of this study was isolation and molecular identification of Streptococcus mutans as an important agent of dental decay and the evaluation of antimicrobial effect of herbal medicine on them. Materials and methods: To perform the study 50 oral samples were collected from ordinary people and cultivated on the nutrient agar plates. In addition, 27 herbal medicine plants were purchased and water and hydro alcoholic extracts of them were prepared. Then, the antimicrobial effects of water and hydro alcoholic extracts of herbal medicine plants were evaluated on the isolates. In addition, MIC, MBC and Arbitrary units of the effective herbal medicine plants were determined. Results: The results indicated that Streptococcus was predominant oral microflora and Streptococcus mutans were isolated in high frequency. Out of all herbal plants, Mazoo plant, oak plant, Myrtus commuis and Tribulus terrestris exhibited high antimicrobial property on Streptococcus mutans isolates. However, hydroalcolic Mazoo plant extract showed relatively more antimicrobial effect. Furthermore, MIC, MBC and Arbitrary unit of the Mazoo plant extract was relatively less. Hence, our finding showed antimicrobial effect of Mazoo plant extracts on oral microbiota viz., Streptococcus mutans. Conclusion: Therefore, consumption of aliquot of hydroalcolic Mazoo plant extract can be eliminated oral infections as well as dental decay. However, it needs more investigation.
Authors and Affiliations
Gholrokh Ansari, Majid Baserisalehi
Keywords
Related Articles
- EP ID EP698427
- DOI -
- Views 79
- Downloads 0
Inhibitory Effect of Hydroalcoholic Extract of Mint Plant on Escherichia ColiIsolated fromLambs and Goat Kidswith Diarrheain Kazeroun
In spite of advances in the management and prevention techniques as well as strategies for diarrhea treatment, it is still a very common and costly disease affecting the small ruminants. The present study aimed at determ...
Investigating the Effects of Shirazi Thyme Extract on Plasma Levels of Testosterone, Dihydrotestosterone, LH and FSH in Adult Male Wistar Rats: An Experimental Study
Thymus vulgaris contains components that are chemically very different. Some of these components have properties as anticonvulsant, antifungal, antioxidant, anti-inflammatory, anti-infection, anti-diabetics and act as a...
The effect of vitamin E and selenium on treatment process, serum malondialdehyde and vitamin D levels of cows with mastitis
Background and aim: The aim of this research was to investigate the effect of vitamin E and selenium on mastitis treatment outcome and Malondialdehyde (MDA) and vitamin D level in serum. Materials and methods: In this...
The Effect of Tarragon on the Growth of Lactobacillus casei and Lactobacillus paracasei in Probiotic Milk
Probiotics are live microbial food supplements which benefit the health of consumer by maintaining or improving their intestinal microbial balance. This study aimed at investigating the effect of different doses of tarra...
An Investigation into the Effects of Aqueous Extract of Carum Copticum on Renal Tissue and Uric Acid in Male Wistar Rats
Of the nine chemical compounds identified in the Carum Copticum extract, thymol, γ-terpinen, and parasaymn are considered as the main compounds utilized as antiemetic, anti-asthma, anti-nausea, and spasmodic, locally use...