EVALUATION OF TOXICITY OF BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES (Ag-NPs) USING LEMNA TEST AND ALGALTOXKIT F
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2016, Vol 40, Issue 330
Abstract
In the current study the toxicity of biologically synthesized Ag-NPs to Selenastrum capricomutum and Lemna minor was assessed. The supernatants from the Bacillus subtilis cultures were applied for the Ag-NPs synthesis. The following media were used for culturing of Bacillus subtillis (I’-1a): molasses (Mol.), brewery effluent (# 6) and Luria-Bertani (LB) medium. Silver nitrate was added to the culture supernatants to the final concentration of 1 mM. The Ag-NPs presence was monitored by UV-Vis spectrophotometer. Toxicity tests were made according to OECD Guideline 201 (2006) and ISO standard 20079 (2006). In the test AlgaltoxkitF the two parameters cell growth and content of chlorophyll a were evaluated. However, in the test with Lemna minor the five endpoints including: number and total area of fronds, fresh weight (f.w.), chlorophylls a (Chl a) and b (Chl b) were used to assess phytotoxicity. Ag+ ions were considered to be the most toxic for Selenastrum capricornutum and Lemna minor than Ag-NPs biologically synthesized. The toxicity of biologically synthesized Ag- NPs was lower to S. capricomutum and to L. minor for all analyzed parameters compared to the toxicity of Ag+. However, the toxicity of Ag-NPs synthesized in the supernatant from the culture of Bacillus subtilis growing on brewery waste was higher compared to the Ag-NPs synthesized in the supernatants from the cultures of strain growing on molasses and LB media. The variability in sensitivity of both organisms towards Ag-NPs was observed.
Authors and Affiliations
Joanna CHOJNIAK, Agata DROBNIEWSKA, Łukasz JAŁOWIECKI, Izabela BIEDROŃ, Grażyna PŁAZA, Grzegorz NAŁĘCZ-JAWECKI
Keywords
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