“Evaluation ofPCR – Based Techniques fortheDetection ofMycobacterium Tuberculosis Complex inSputum Samples”
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 6
Abstract
Tuberculosis has been one of the major causes of death globally. Due to the high risk of contact transmission. This has led to the increase in the morbidity and mortality rates and several countries have been endemic, especially in the developing countries. Ranked as the second leading cause of morbidity from communicable diseases after HIV/AIDS. Study evaluated the Mycobacterium T. complex from sputum samples and perform PCR using IS6110 gene sequence to detect MTB complex. Crossectional study design with sample of 100 collected from the OPD and IPD conveniently at the department of micro-biology. Study period was from Aug., 2012 to Aug., 2015. Sputum samples for smear culture was collected as per the RNTCP guidelines. About 70% of the males and 30% of females were susceptible. Of which at 72weeks reported 86% with cough. Those with severe fever were about 73%, chest pain 60%, breathlessness 70% and loss of appetite 78%. Among the 100 sample in the ZN staining, 14% was positive and 86% negative. In the PCR sputum sample, 67% was negative and 33% positive. On comparing ZN smear positive and PCR, the sensitivity of PCR test was 100% in smear positive samples. While smear negative were also positive in PCR and sensitivity of PCR was 20.9%. In conclusion, isolation of 100 samples using microscopy and PCR indicated that there were discordance in 19% cases. Great care should be taken in designing primer pairs for the insertion sequence IS6110 to avoid false negative or false positive results. Pulmonary T. patients are more likely to be present with the specific symptoms. The yield of sputum smear positive Pulmonary T. cases can be improved if patients with more than 2weeks history of cough are screened to diagnose such cases. Therefore there is urgent need for modern techniques especiallyin developing countries to facilitate better outcome of diagnosis and public health control measures.
Authors and Affiliations
Ajabsingh Chaudhary, Dr. Ajoke B. Akinola, Prof. Dr. Dakshina Bisht
Keywords
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- EP ID EP376503
- DOI 10.9790/0853-1706071622.
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