Evalution of reactive oxygenproduction using whole blood samples by chemiluminescence
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 2
Abstract
Conventionally, it has been necessary to isolate neutrophils to measure the production capacity of reactive oxygen species (ROS), and isolation takes a long time due to a large amount of blood. Although cytometry has been applied to measure ROS in a whole blood preparation, special equipment is required. We therefore investigated the feasibility of using a chemiluminescence technique to rapidly measure ROS in whole blood. Chemiluminescent reagents 8-amino-5-chloro-7-phenylpyrido (3,4-d) pyrodazine-1,4 (2H,3H) dione (L-012), 2-Methyl-6-p-methoxyphenylethynylimidazopyrazinone (MPEC), luminol, and lucigenin, as well as the stimulants phorbol myristate acetate (PMA), formil-methionyl-leucyl-phenylalanine (fMLP), and opsonuzed zymosan (OZ) were used in the present study, and emission intensities were captured using a Luminescencer PSN. The results showed that L-102 exhibited a high degree of reaction specificity toward hypochlorous acid (HOCl) and sufficient sensitivity in the analysis using whole blood. Because this technique enables rapid measurement of ROS in trace samples, it is expected to be a valuable clinical laboratory assay.
Authors and Affiliations
Kaoru Akiyama
Keywords
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