Evidence of a Reproduction-Related Function for Brine Kisspeptin2 and its Receptors in Fish Trichogaster trichopterus
Journal Title: International Journal of Zoological Investigations - Year 2017, Vol 3, Issue 2
Abstract
This paper describes the transcription of Kisspeptin2 (Kiss2) and Kiss receptors (GPR54 or Kiss2r, Kiss1r) in blue gourami (Trichogaster trichopterus), which belongs to the suborder Anabantoidei and family Osphronemidae. A low degree of identity cDNA acid sequences was found in homologous partial sequences Kiss2 and Kiss1r from a number of fish species as compared to the blue gourami sequence, however, the variation in Kiss2r and other fish species is much lower. In order to determine whether the Kiss2, Kiss1r and Kiss2r genes are expressed in the brain of females, total RNA were reverse-transcribed using qPCR amplification in comparison to the Beta-Actin gene. Relative mRNA levels of Kiss2, Kiss1r and Kiss2r were determined in brains excised from females before vitellogenesis (previtellogenic) (PV) and during vitellogenesis (VT) in mature females. The differences found between Kiss2, Kiss2r, juvenile and mature females, were statistically significant. There was no significant difference between PV and VT in the mRNA levels of Kiss1r. In comparing the transcription of Kiss1r to Kiss2r and Kiss2 mRNA levels, we found significant differences in transcription levels in both juvenile and mature females between the genes. We proposed a quality model showing the Kiss2 mechanism involved in regulating vitellogenesis in blue gourami.
Authors and Affiliations
Gad Degani
Keywords
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