EXAGGERATION OF TYPE 2 DIABETES DUE TO CAFFEINE-NICOTINE CO-ADMINISTRATION: A STUDY IN RATS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 9
Abstract
Objective: This study evaluated the toxic effect of simultaneously injected normal doses of caffeine and nicotine in diabetic lab animals.Methods: A study was conducted for three weeks in seven rat groups (n=6); viz. first non-diabetic group treated with caffeine (20 mg/kg, ip) twice daily, second with nicotine (0.4 mg/kg, ip) twice daily and third with both treatments simultaneously; whereas other three groups treated in the same way but inducing diabetes; and employing the seventh group as diabetic control. Type 2 diabetes was induced by high fatty diet prior for two weeks and a single streptozotocin injection on 1th day of study in all diabetic groups. Blood and urine samples were collected weekly to estimate blood parameters. Animals were sacrificed, and organs were collected for histopathology analysis.Results: Most blood parameters showed a rapid increase in diabetes in co-addiction group compared with their single addiction or non-addiction control groups. Caffeine-nicotine co-addiction group showed about 60-80 mg/dl (p<0.05) rise in serum glucose, 15-20 U/l in AST (p<0.01), 80-100 U/l in ALT (p<0.01), 20-30 mg/dl in Urea (p<0.01), 02 mg/dl in creatinine (p<0.05), 12-15 mg/dl (p<0.01) in LDL-C, 6-9 mg/dl in VLDL-C (p<0.01) and 60-90 mg/dl in TC levels (p<0.01) when compared with non-addicted diabetic control. There was a significant reduction in HDL-C (p<0.01) while the less significant rise in triglycerides in the case of co-addiction as compared to non-addiction diabetic control group. Histopathology results exhibited moderate to severe tissue damage in agreement with clinical biochemistry results.Conclusion: Nicotine-caffeine co-addiction harms exceptionally more in type 2 diabetes greater than their single addiction or non-addiction.
Authors and Affiliations
Subhash T. Kumbhar, Hemant D. Une, Anagha M. Joshi, Pralhad B. Wangikar
Keywords
Related Articles
- EP ID EP576047
- DOI 10.22159/ijpps.2016.v8i9.13590
- Views 71
- Downloads 0
COMPARATIVE STUDY OF AQUEOUS EXTRACT OF MOMORDICA CHARANTIA SEEDS WITH SYNTHETIC INSULIN SENSITIZERS ON BLOOD GLUCOSE LEVELS AND BODY WEIGHT IN ALBINO RATS
Objective: To evaluate the effect of aqueous extract of Momordica charantia seed in combination with an insulin sensitizer metformin on blood glucose levels and body weight compared to metformin plus pioglitazone. Method...
MICROWAVE ASSISTED GREEN SYNTHESIS OF PHARMACEUTICALLY IMPORTANT DIHYDROPYRIMIDINONES IN FRUIT JUICE MEDIUM
Objective: The objective of this research project was to perform the green synthesis of a series of Dihydropyrimidinone (DHPM) derivatives which are having immense importance in biological and pharmaceutical science, via...
EPIDEMIOLOGY AND OUTCOME AMONG SEVERE SEPSIS AND SEPTIC SHOCK PATIENTS IN A SOUTH INDIAN TERTIARY CARE HOSPITAL
Objective: The objective of this research was to determine the prevalence of severe sepsis and septic shock and evaluate its outcome.Methods: This was a prospective, observational study, in which adult patients with seve...
ANTIBACTERIAL ACTIVITY OF FRESH WATER CRAB AND SNAIL AND ISOLATION OF ANTIBACTERIAL PEPTIDES FROM HAEMOLYMPH BY SDS – PAGE
Objective: The present study was undertaken to characterize antimicrobial molecules from the fresh water snail and crab.Methods: Collection of haemolymph, preparation of extracts, antimicrobial activity, TLC analysis, SD...
ANTIHYPERGLYCEMIC, ANTIOXIDANT, AND PANCREAS REGENERATION ACTIVITIES OF BLACK CUMIN (NIGELLA SATIVA L.) SEEDS ETHANOL EXTRACT IN ALLOXAN-INDUCED DIABETIC RATS
Objective: The goal of this study was to determine the antihyperglycemic, antioxidant as well as pancreas regeneration effects of ethanol extracts of Nigella sativa L. seeds in alloxan-induced diabetic rats.Methods: The...