Experimental study of the cytotoxic effects of neutrophils on chondrocytes cultivated in different oxygen concentrations
Journal Title: Reumatologia - Year 2006, Vol 44, Issue 2
Abstract
The main objective of our work was to examine how different oxygen levels affects cytotoxic interactions between human neutrophils and chondrocytes obtained from the cartilage. We studied the connections between neutrophils activation (CL) and the availability of glucose, the amount of energy and the activity of the penthose phosphate pathway. We found that NADPH oxidase affects the intensity of the inflammation process but the amount of energy (ATP) had no direct influence on neutrophils activation. Chondrocytes cultivation in different oxygen conditions demonstrated that although in high oxygen levels (21%), the survival of the cells being in hyperoxia- state varies not from cells cultivated in low oxygen conditions, they are significantly more exposed to the toxic influence of neutrophils. The inactivation of NADPH production, noticeably lowering the level of damage delivered to the chondrocytes, could suggest that a blocker of this pathway (DOG) might be a useful remedy, reducing the intensity of the inflammation process.
Authors and Affiliations
Michał Gajewski, Ewa Warnawin, Joanna Gajewska, Grażyna Sygitowicz, Maria Rell-Bakalarska, Tomasz Burakowski, Wojciech Dziewczopolski, Szymon Szczepanik, Łukasz Wysocki, Jan Pachecka, Włodzimierz Maśliński, Sławomir Maśliński
Keywords
Related Articles
- EP ID EP82364
- DOI -
- Views 100
- Downloads 0
Najnowsze poglądy na temat leczenia zesztywniającego zapalenia stawów kręgosłupa
Zesztywniające zapalenie stawów kręgosłupa (ZZSK) jest przewlekłą chorobą zapalną, prowadzącą do upośledzenia funkcji i niepełnosprawności. ZZSK jest związane ze znacznymi bezpośrednimi i pośrednimi kosztami socjoekonomi...
In-patients as a source of medical information for patients admitted to the rheumatological ward
One hundred patients admitted to the rheumatological ward were questioned concerning the will to discuss their medical problems with other in-patients and the impact of such obtained data on their attitude to medical pro...
Efficacy and safety of TNF inhibitors - results of randomized controlled clinical trials
TNF inhibitors were first approved for treatment of active rheumatoid arthritis (RA) in 1998. Since that time, a series of multicentre, randomized controlled trials have been evaluated in early and established RA, in p...
Ochronotic spondylosis of 5 years old boy - a case report
Alkaptonuria is a rare disorder caused by congenital homogentisate oxidaze deficiency of recessive inheritance. Homogentisate polymers are accumulated and manifested with urine darkening and ochronosis. A 12 years case...
Zebranie Komitetu ds. Edukacji Europejskiej Ligi do Walki z Chorobami Reumatycznymi (EULAR/SCET) Amsterdam, 21 czerwca 2006 r.