Explant combined with trypsin-digestion for culture of endothelial cells from infantile hemangiomas
Journal Title: Di-er Junyi Daxue Xuebao - Year 2009, Vol 30, Issue 2
Abstract
Objective:To explore a novel method for cultivation of the endothelial cells(EC) from infantile hemangiomas(IH) in vitro and observe the biological character of cultured endothelial cells.Methods:Fresh operative specimens were obtained from infantile hemangiomas.The endothelial cells of IH were cultured by explant combined with trypsin-digestion technique.Immunohistochemical staining of EnVision method was carried out to identify the cultured cells.The purity of endothelial cell was examined by flow cytometry analysis of FITC-CD34.The biologic characters of endothelial cells were observed under inverted phase contrast microscope.Results:Endothelial cells were successfully cultured from 8 IH specimens of all the 14 explants.The morphology of cultured endothelial cells included two types:polygonal cells and fusifourm cells.The cultured cells were homogenously positive for EC markers-vWF or CD34,indicating their EC origin.Tube formation was found in endothelial cells of IH.The proportion of CD34+ cells was 76.28% in cultured endothelial cells as detected by flow cytometry analysis.Conclusion:ECs can be successfully isolated and cultured from infantile hemangiomas by explant combined with trypsin-digestion technique; the cultured cells have some characters of endothelial cells.
Authors and Affiliations
Hong-yue LIAO
Keywords
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